PMID: 6400919Oct 1, 1984Paper

A comparison of different nutrient media and supplementation with dexamethasone for mouse colon organ culture

Cell Biology and Toxicology
B Reiss, G M Williams

Abstract

Several complex nutrient media were compared for their effectiveness in maintaining viable and functional mouse colon mucosa in organ culture. The order of superiority for preserving survival of normal tissues for 14 days was: Williams' Medium E greater than Morgan's 199 greater than CMRL-1066 greater than Waymouth's MB 752/l greater than Eagle's MEM greater than Trowell's T8. The 3H-thymidine labeling index was highest in colon explants maintained in Morgan's 199 greater than Williams' Medium E greater than Waymouth's MB 752/l greater than CMRL-1066 greater than Eagle's MEM. However, the very high labeling produced by Morgan's 199 medium was abnormal in comparison to in vivo levels. Supplementation with 1.0 uM dexamethasone almost always improved crypt survival and maintained normal DNA synthetic activity.

References

Jan 1, 1975·In Vitro·L J Schiff
Jan 1, 1976·Methods in Cell Biology·G M Williams
Sep 12, 1979·Biochemical and Biophysical Research Communications·B J Blaauboer, A J Paine
Mar 1, 1978·Proceedings of the Society for Experimental Biology and Medicine·B T MossmanJ E Craighead
Dec 1, 1978·Pathology, Research and Practice·A K ShamsuddinB F Trump
Nov 1, 1974·Experimental Cell Research·G M Williams, J M Gunn
Oct 1, 1980·In Vitro·L J Schiff, S J Moore
Jan 1, 1982·Carcinogenesis·S S HechtG M Williams
Dec 1, 1982·Journal of Biochemistry·Y HoriutiA Ichihara

❮ Previous
Next ❯

Citations

Sep 1, 1986·Cell Biology and Toxicology·J H ResauC Newkirk
Nov 1, 1991·Cytotechnology·J H ResauS J Meltzer
Nov 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·T L RissD A Sirbasku
Dec 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·T L Riss, D A Sirbasku
Sep 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M Ogasawara, D A Sirbasku
Nov 13, 2008·Cytotechnology·Joanne KeenanMartin Clynes
Jan 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·W L McKeehanG H Sato

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.