PMID: 3772109Oct 23, 1986Paper

A comparison of ELISA screening methods for the production of monoclonal antibodies against soluble protein antigens

Journal of Immunological Methods
D M BrennandD W Hough

Abstract

A library of monoclonal antibodies against pig heart citrate synthase has been raised. Twelve solid-phase immunoassay systems, employing different methods of antigen immobilization, have been compared for their ability to detect the various members of this library. It was found that a sandwich immunoassay, in which the citrate synthase antigen was immobilized on the solid support via a polyclonal antibody preparation, was the only system capable of detecting all the monoclonal antibodies tested. It is suggested that such a sandwich assay should be used in preference to direct assays for the initial screening of monoclonal antibodies.

References

Apr 22, 1985·Journal of Immunological Methods·G Gagnon, Y Raymond
Aug 1, 1983·Analytical Biochemistry·A L De Blas, H M Cherwinski
Jan 1, 1980·Journal of Immunological Methods·S F de StGroth, D Scheidegger
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon

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Citations

May 8, 1990·Journal of Immunological Methods·K CarrollR O'Kennedy
Feb 3, 1993·Journal of Immunological Methods·S C SmithK James
Apr 12, 2000·Journal of Immunological Methods·M Steinitz, L Baraz
Apr 9, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·G PatelD M Brennand

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