A comparison of fluorescein diacetate and propidium iodide staining and in vitro excystation for determining Giardia intestinalis cyst viability.

Parasitology
A L Smith, H V Smith

Abstract

The viability of 4 human isolates of Giardia intestinalis cysts using either the fluorogenic vital dyes fluorescein diacetate (FDA) and propidium iodide (PI) or in vitro excystation was assessed. Whereas viable cysts, as defined by in vitro excystation were present in each of the 4 isolates, cysts from only 3 of the 4 isolates took up the vital dyes. FDA consistently over-estimated cyst viability whilst PI under-estimated non-viable cysts when compared with in vitro excystation. Following in vitro excystation, both FDA and PI stained a proportion of unexcysted cysts indicating that FDA stained cysts which were incapable of excystation, whereas PI did not stain all cysts which were incapable of excystation. One human cyst isolate, which underwent in vitro excystation, could not be stained with either FDA or PI. In the absence of currently more specific fluorescent indicators of viability, PI alone could be used to determine the lower limit of nonviability in positive water-related samples, where small numbers of cysts are to be expected.

References

Jan 1, 1977·Journal of Immunological Methods·P K Horan, J W Kappler
Jan 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K H Jones, J A Senft

❮ Previous
Next ❯

Citations

Jun 1, 1996·International Journal for Parasitology·R Taghi-KilaniM Belosevic
Jul 1, 1997·International Journal for Parasitology·M BelosevicG R Finch
Mar 1, 2002·Journal of Microbiological Methods·Walter Quintero-BetancourtJoan B Rose
Feb 20, 2010·PLoS Neglected Tropical Diseases·Hugo Aguilar-DíazJulio C Carrero
Sep 9, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Taís Rondello Bonatti, Regina Maura Bueno Franco
Apr 25, 2013·MicrobiologyOpen·Carlos A NiñoMoises Wasserman
Feb 19, 2015·Luminescence : the Journal of Biological and Chemical Luminescence·Samuel Ricardo dos SantosCristiano de Mello Gallep
Nov 6, 2013·Biotechnology Advances·Anson V KoehlerRobin B Gasser
Jan 1, 2014·International Scholarly Research Notices·José Roberto GuimarãesLuciana Urbano Dos Santos
May 13, 2015·European Journal of Protistology·Anastasia ShatilovichElizaveta Rivkina
Sep 10, 2011·Veterinary Parasitology·G H GritT Geurden
Nov 30, 2004·Cell Transplantation·M J BarnettJ R T Lakey
Dec 18, 2003·Journal of Applied Microbiology·C DaltonH V Smith
Aug 1, 1992·Applied and Environmental Microbiology·M Y Deng, D O Cliver
Jan 1, 1997·Applied and Environmental Microbiology·M AbbaszadeganI L Pepper
Nov 1, 1991·Applied and Environmental Microbiology·J F SauchW Jakubowski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.