A comparison of four methods for determining viability in human dermal fibroblasts irradiated with blue light

Journal of Pharmacological and Toxicological Methods
Daniela S Masson-MeyersChukuka S Enwemeka

Abstract

Several tests are available for assessing the viability of cells; however, there is a dearth of studies comparing the results obtained with each test. We compared the capability of four viability assays (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT), neutral red, trypan blue and live/dead fluorescence), to detect potential toxicity in fibroblasts irradiated with 470nm blue light. Cells were irradiated at 3, 55, 110 and 220J/cm(2), incubated for 24h and viability assessed using each test. MTT assay showed significant decreases in viability when cells were irradiated with 110 and 220J/cm(2) energy fluence (dose) (89% and 57% viable cells, respectively; p<0.0001, compared to control); likewise the trypan blue assay showed 42% and 46% viable cells (p<0.0001). Neutral red assay revealed significant decrease in viability when cells were irradiated with 220J/cm(2) (84% viable cells; p=0.0008, compared to control). The live/dead fluorescence assay was less sensitive, evincing 91% and 95% viable cells after irradiation with 110 and 220J/cm(2) respectively. (1) The four assays differed in their levels of sensitivity to cell viability. (2) The adverse effect of increasing doses seems to manifest as alteration of mito...Continue Reading

References

Aug 1, 1989·Journal of Photochemistry and Photobiology. B, Biology·T Karu
Aug 1, 1989·Journal of Photochemistry and Photobiology. B, Biology·S Passarella
Jan 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K H Jones, J A Senft
Jan 9, 1998·Photochemistry and Photobiology·W YuR J Lanzafame
Jul 21, 2000·International Journal of Radiation Biology·D PastoreS Passarella
Feb 3, 2004·Journal of Cell Science·J Michael Sorrell, Arnold I Caplan
May 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R SethE A Roberts
Dec 29, 2004·International Journal of Pharmaceutics·Jörg WeyermannAndreas Zimmer
Mar 2, 2005·Lasers in Surgery and Medicine·Tiina I KaruNatalia I Afanasyeva
Mar 31, 2005·The Journal of Biological Chemistry·Bernard F GodleyMike Boulton
Apr 26, 2007·Mutagenesis·Michael D Fellows, Michael R O'Donovan
Feb 20, 2008·Mutagenesis·Andrew R CollinsRudolf Stetina
Dec 10, 2008·Lasers in Surgery and Medicine·Chukuka S EnwemekaSombiri K Enwemeka
Feb 7, 2009·Photomedicine and Laser Surgery·Chukuka S EnwemekaDavid Yens
Feb 10, 2009·Applied and Environmental Microbiology·Michelle MacleanGerry Woolsey
Aug 14, 2009·The Journal of Investigative Dermatology·Joerg LiebmannVictoria Kolb-Bachofen
Mar 18, 2010·Photomedicine and Laser Surgery·Deise A A Pires-OliveiraCristina Pacheco-Soares
Apr 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·L VianJ P Cano
Mar 23, 2011·Journal of Photochemistry and Photobiology. B, Biology·Christian OpländerChristoph V Suschek
Aug 3, 2011·Journal of Biomedical Optics·Luiz Evaristo Ricci VolpatoMaria A A M Machado
Jul 31, 2012·International Journal of Dentistry·Fernanda G BassoCarlos A de Souza Costa
Oct 22, 2013·Photomedicine and Laser Surgery·Chukuka S Enwemeka
Apr 9, 2014·Archives of Toxicology·Aylin UstündağGisela H Degen
Feb 24, 2015·Lasers in Medical Science·Violet Vakunseh BumahChukuka Samuel Enwemeka
Sep 13, 2015·Lasers in Medical Science·Daniela Santos Masson-MeyersChukuka Samuel Enwemeka

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Citations

Apr 20, 2016·Journal of Photochemistry and Photobiology. B, Biology·Daniela Santos Masson-MeyersChukuka Samuel Enwemeka
Oct 3, 2018·Lasers in Medical Science·José de Alencar Fernandes NetoMaria Helena Chaves de Vasconcelos Catão
Mar 18, 2020·Molecular Biology Reports·Jagdish Gopal PaithankarRajashekhar K Patil
Jan 25, 2021·Lasers in Medical Science·Violet Vakunseh BumahChukuka Samuel Enwemeka
Nov 18, 2017·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Yucheng WangTianhong Dai
Jun 12, 2021·The Journal of Investigative Dermatology·Henry W LimThierry Passeron

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