A comparison of human and porcine acellularized dermis: interactions with human fibroblasts in vitro

Plastic and Reconstructive Surgery
Alexis D ArmourJohn L Semple

Abstract

Dermal substitutes derived from xenograft materials require elaborate processing at a considerable cost. Acellularized porcine dermis is a readily available material associated with minimal immunogenicity. The objective of this study was to evaluate acellularized pig dermis as a scaffold for human fibroblasts. In vitro methods were used to evaluate fibroblast adherence, proliferation, and migration on pig acellularized dermal matrix. Acellular human dermis was used as a control. Pig acellularized dermal matrix was found to be inferior to human acellularized dermal matrix as a scaffold for human fibroblasts. Significantly more samples of human acellularized dermal matrix (83 percent, n = 24; p < 0.05) demonstrated fibroblast infiltration below the cell-seeded surface than pig acellularized dermal matrix (31 percent, n = 49). Significantly more (p < 0.05) fibroblasts infiltrated below the surface of human acellularized dermal matrix (mean, 1072 +/- 80 cells per section; n = 16 samples) than pig acellularized dermal matrix (mean, 301 +/- 48 cells per section; n = 16 samples). Fibroblasts migrated significantly less (p < 0.05) distance from the cell-seeded pig acellularized dermal matrix surface than in the human acellularized derm...Continue Reading

References

Apr 1, 1979·Plastic and Reconstructive Surgery·N M BreachA Yiacoumettis
Dec 1, 1992·Burns : Journal of the International Society for Burn Injuries·Z R GaoR J Spence
Jan 1, 1982·Biomaterials·R F OliverR A Grant
Jun 1, 1995·Burns : Journal of the International Society for Burn Injuries·D J Wainwright
Jun 1, 1994·Journal of Biomedical Materials Research·D W CourtmanG J Wilson
Apr 1, 1993·Burns : Journal of the International Society for Burn Injuries·E MatouskováR Königová
Jun 1, 1997·The British Journal of Dermatology·F Berthod, O Damour
Oct 27, 1997·Annals of Plastic Surgery·M M GhoshS Mac Neil

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Citations

Aug 2, 2007·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Gresham T RichterEmre Vural
Jul 26, 2008·Tissue Engineering. Part a·Janet E ReingStephen F Badylak
Oct 23, 2009·Tissue Engineering. Part a·Nicola GreenJonathan P Bury
Nov 1, 2012·Plastic and Reconstructive Surgery·Chandra V Ellis, David A Kulber
Feb 18, 2011·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·William G StebbinsJeffrey Petersen
Oct 9, 2013·World Journal of Surgery·Ali DarehzereshkiNamir Katkhouda
Nov 29, 2007·Journal of Biomedical Materials Research. Part a·Xuepeng XiongSangang He
Nov 26, 2010·The Annals of Thoracic Surgery·Phillip S GeTimothy L Van Natta
Mar 29, 2014·Journal of Tissue Engineering and Regenerative Medicine·Timothy J Keane, Stephen F Badylak
Mar 26, 2015·Journal of Materials Science. Materials in Medicine·Guodong SongHaibin Zuo
Aug 28, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Ekaterina VorotnikovaSusan J Braunhut
Dec 7, 2011·Surgical Innovation·Neil J Smart, Stephen Bloor
Sep 14, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Isarawut PrasertsungSiriporn Damrongsakkul
Dec 22, 2019·Veterinary Surgery : VS·Omar G El-TaliawiDominique J Griffon
Aug 25, 2016·Plastic and Reconstructive Surgery·Mayer Tenenhaus, Hans-Oliver Rennekampff
Nov 20, 2019·Annals of Biomedical Engineering·Madeline C Cramer, Stephen F Badylak
Jun 19, 2021·Journal of the American Podiatric Medical Association·Jenna C BekenyChristopher E Attinger

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