A comparison of in vivo and in vitro neuroimaging of 5-HT 1A receptor binding sites in the cat brain

Journal of Chemical Neuroanatomy
Nicolas AznavourLuc Zimmer

Abstract

To validate the cat as a suitable model for positron emission tomography imaging (PET) and to gain further knowledge on the anatomical distribution of the serotonin-1A receptor (5-HT 1A) in the feline brain, we used PET with [18F]MPPF and in vitro autoradiography with [3H]MPPF, [3H]8-OH-DPAT and [3H]paroxetine. PET radioactivity curves with [18F]MPPF were very reproducible in anaesthetized cats, with the highest radioactivity uptakes recorded in the hippocampus, cingulate cortex, septum, infralimbic cortex and raphe nucleus, whereas the lowest were found in the cerebellum. [3H]8-OH-DPAT binding displayed a comparable, albeit lower, regional distribution than with [3H]MPPF. Autoradiography also revealed the presence of 5-HT 1A receptor binding sites in the cortex and in the interpeduncular nucleus, due to its greater sensitivity and spatial resolution compared with PET imaging. The cat constitutes an interesting experimental model for PET imaging, as many physiological concepts have been well established with this animal. Our study also shows the advantages of combining complementary neuroimaging techniques such as in vivo PET imaging and in vitro autoradiography to visualize the distribution of the 5-HT 1A receptors.

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Citations

Aug 16, 2008·European Journal of Nuclear Medicine and Molecular Imaging·Nicolas AznavourLaurent Descarries
Oct 1, 2009·European Journal of Nuclear Medicine and Molecular Imaging·Laëtitia LemoineLuc Zimmer
Sep 11, 2007·Drug Discovery Today·J S Dileep Kumar, J John Mann
Nov 15, 2006·Neuropharmacology·Nicolas Aznavour, Luc Zimmer
Jul 29, 2008·European Journal of Pharmacology·Lydia GüntherJochen Oehler
Mar 3, 2009·Neurobiology of Aging·Mathieu VerdurandLuc Zimmer
Mar 23, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Albertine DuboisThierry Delzescaux
Jul 22, 2014·CNS Neuroscience & Therapeutics·Guillaume BeckerLuc Zimmer
Jul 22, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Benjamin VidalLuc Zimmer
May 12, 2012·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Laëtitia LemoineLuc Zimmer

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