PMID: 7514667Feb 15, 1994Paper

A comparison of non-NMDA receptor channels in type-2 astrocytes and granule cells from rat cerebellum

The Journal of Physiology
D J Wyllie, S G Cull-Candy

Abstract

1. Patch-clamp recording methods have been used to compare the pharmacological properties and single-channel characteristics of non-NMDA receptor channels in cerebellar type-2 astrocytes and granule cells. 2. In type-2 astrocytes whole-cell concentration-response curves for glutamate, quisqualate, AMPA and kainate gave EC50 values of 5.8, 3.8, 7.6 and 160 microM and Hill slopes of 1.65, 1.18, 1.64 and 1.65, respectively, resembling estimates for granule cell receptors. 3. The non-NMDA receptor antagonists CNQX and diCl-HQC (see Methods) inhibited whole-cell kainate currents in both cell types. The IC50 for CNQX antagonism of the kainate response was 536 nM in type-2 astrocytes, and 500 nM in granule cells. The IC50 for diCl-HQC was 3.5 microM in astrocytes and 3.7 microM in granule cells. 4. CNQX acted as a competitive antagonist of whole-cell kainate responses in type-2 astrocytes and granule cells giving Schild plots with a slope near 1. The equilibrium constant, K, for CNQX binding was 524 nM in astrocytes and 489 nM in granule cells. 5. Quisqualate and AMPA responses showed rapid desensitization in type-2 astrocytes with a ratio of steady-state to peak response of 0.09. Concanavalin A reduced this desensitization. 6. Non-NM...Continue Reading

Citations

Mar 21, 2015·Neuroscience·C R Rose, J-Y Chatton
Jul 1, 1995·Brain Research. Molecular Brain Research·N C DayP J Shaw
Aug 6, 2005·Experimental Brain Research·Troy L Carter, James G McElligott
Sep 1, 1995·The European Journal of Neuroscience·G Seifert, C Steinhäuser
Aug 1, 1995·Current Biology : CB·S Cull-Candy
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Sharma, S Vijayaraghavan
Apr 27, 2000·Neuroscience and Biobehavioral Reviews·P R LamingK Ng
Dec 16, 2011·The Journal of Pharmacology and Experimental Therapeutics·Xueliang FanEllen J Hess
Nov 1, 2001·Current Protocols in Toxicology·J Oberdoerster
Feb 11, 1998·Physiological Reviews·A VerkhratskyH Kettenmann
Jul 1, 1997·Brain Research. Molecular Brain Research·G SeifertC Steinhäuser
Apr 13, 2000·Brain Research. Brain Research Reviews·A Verkhratsky, C Steinhäuser

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