A comparison of ovine monocyte-derived macrophage function following infection with Mycobacterium avium ssp. avium and Mycobacterium avium ssp. paratuberculosis

Immunology and Cell Biology
Sven T Berger, Frank T Griffin

Abstract

Mycobacterium avium ssp. paratuberculosis causes Johne's disease in ruminants, whereas the antigenically and genetically similar subspecies Mycobacterium avium ssp. avium is less virulent. In this study, we compared one strain of each subspecies for its ability to survive, induce cytokines, suppress MHC class I and II expression and induce apoptosis or necrosis in ovine monocyte-derived macrophages. Both subspecies survived intracellularly and induced the secretion of IL-10. Low levels of TNF-alpha were detected after infection with both subspecies at 4 h. IL-12 was not upregulated after infection. Downregulation of MHC class I and II was evident in response to infection with both M. avium ssp. avium and M. avium ssp. paratuberculosis. No significant cytotoxicity was detectable in ovine macrophages after the addition of bacteria. M. avium ssp. paratuberculosis induced slightly more apoptosis than M. avium ssp. avium. Still the overall rate of apoptosis was very low and both subspecies suppressed LPS-induced macrophage apoptosis.

References

Jan 13, 1995·Journal of Immunological Methods·A LévesqueM Pagé
Jan 1, 1996·Veterinary Immunology and Immunopathology·H M AlzuherriC J Clarke
Apr 1, 1997·Journal of Comparative Pathology·C J Clarke
Nov 22, 1997·Journal of Immunological Methods·G M Graziani-BoweringL G Filion
Jan 16, 1998·Research in Veterinary Science·H M AlzuherriC J Clarke
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J KeaneH Kornfeld
Mar 22, 2000·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·H D FladM Ernst
Oct 6, 2001·Veterinary Immunology and Immunopathology·M OlivierF Lantier
Jul 23, 2003·Nature Medicine·Ulrich E SchaibleStefan H E Kaufmann
Apr 29, 2004·Veterinary Immunology and Immunopathology·M S Khalifeh, J R Stabel
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adam J GehringW Henry Boom
May 20, 2005·American Journal of Veterinary Research·Douglas J WeissMitchell S Abrahamsen
Aug 23, 2005·Infection and Immunity·D J BeggJ F T Griffin
Aug 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lingling LiVivek Kapur

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Citations

Sep 8, 2010·Veterinary Immunology and Immunopathology·Kumudika de SilvaRichard Whittington
Dec 3, 2016·Cell Communication and Signaling : CCS·Tariq HussainXiangmei Zhou
Jun 19, 2018·Veterinary Sciences·Garyfalia KarponiEleni Papanikolaou
Sep 10, 2019·PloS One·Katharina WindbichlerJussi Hepojoki
Aug 16, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Clare PridansDavid A Hume

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis