A comparison of rat tracheal transplant models: implantation verses anastomotic techniques for the study of airway rejection

Transplantation
Sang Hyun SungTimothy S Hall

Abstract

In rodent models, investigators have transplanted donor tracheas into a recipient rat's abdomen or s.c. tissue to study airway rejection. We describe a modification of this model, which provides improved histology to study the airway injury related to obliterative bronchiolitis. The standard technique of implanting the donor trachea was compared to a model in which a tracheal Y graft was created by anastomosis of the donor trachea to the recipient airway. Syngeneic and allogeneic tracheal grafts (Lewis and Brown Norway rats) were harvested at 2 and 4 weeks using each model (eight groups). Gross patency at the tracheal anastomosis grafts was 100%. All donor tracheas, which were implanted without an anastomosis, were occluded with mucus (syngeneic) or granulation tissue (allogeneic). Syngeneic implant grafts demonstrated significantly less lumenal granulation tissue 35.3%+/-32 than the allograft implant group (95.3%+/-9.2, P=0.0005 at 4 weeks). The anastomotic allograft group demonstrated significantly less lumenal granulation tissue 48.3%+/-23.7 when compared with the implanted allograft group (P=0.003). The implanted allograft demonstrated a severe loss of epithelial integrity by 2 weeks (16.7%+/-38), which progressed to comple...Continue Reading

References

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Citations

Apr 3, 2004·American Journal of Respiratory Cell and Molecular Biology·Anthony C CruzKenneth C Fang
Aug 12, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M SatoM Liu
Apr 4, 2007·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·M HirschburgerV Grau
Jan 28, 2014·Current Therapeutic Research, Clinical and Experimental·Esra ErtanDerya Umit Talas

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