A comparison of ruminal or reticular digesta sampling as an alternative to sampling from the omasum of lactating dairy cows

Journal of Dairy Science
F FatehiPekka Huhtanen

Abstract

The objective of this study was to develop and compare techniques for determining nutrient flow based on digesta samples collected from the reticulum or rumen of lactating dairy cows with estimates generated by the omasal sampling technique. Pre-experimental method development suggested, after comparing with the particle size distribution of feces, application of primary sieving of ruminal and reticular digesta from lactating cows through an 11.6-mm sieve, implying that digesta particles smaller than this were eligible to flow out of the rumen. For flow measurements at the different sampling sites 4 multiparous, lactating Nordic Red cows fitted with ruminal cannulas were used in a Latin square design with 4 dietary treatments, in which crimped barley was replaced with 3 incremental levels of protein supplementation of canola meal. Digesta was collected from the rumen, reticulum, and omasum to represent a 24-h feeding cycle. Nutrient flow was calculated using the reconstitution system based on Cr, Yb, and indigestible neutral detergent fiber and using (15)N as microbial marker. Large and small particles and the fluid phase were recovered from digesta collected at all sampling sites. Bacterial samples were isolated from the diges...Continue Reading

References

Sep 1, 1992·Journal of Dairy Science·G A Broderick, N R Merchen
Nov 1, 1986·The British Journal of Nutrition·T MichalowskiG Breves
Jul 1, 1997·Journal of Dairy Science·D R Mertens
Aug 1, 1997·Journal of Animal Science·D L Harmon, C J Richards
Jul 8, 2003·The British Journal of Nutrition·Seppo AhvenjärviPekka Huhtanen
Jan 1, 1964·The British Journal of Nutrition·A M DOWNES, I W MCDONALD
Jan 9, 2013·Journal of Dairy Science·R MartineauH Lapierre

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