A Comparison of Techniques for Collecting Skin Microbiome Samples: Swabbing Versus Tape-Stripping

Frontiers in Microbiology
Kazuhiro OgaiShigefumi Okamoto

Abstract

The swabbing and tape-stripping methods have traditionally been used for collecting skin microbiome samples for skin bacterial analysis, although no reports have compared the outcome of these methods for collecting skin bacteria. Our purpose was to show the differences in microbial composition between samples collected using the swabbing and tape-stripping methods, by both the next generation sequencing and culture studies. The skin microbiome was collected by both methods, and the samples were processed for a sequence-based microbiome analysis and culture study. The next-generation sequencing results showed that skin bacteria collected using the tape-stripping method were comparable to those collected using the swabbing method. In the culture study, the tape-stripping method collected a greater number and wider variety of viable skin bacteria than the swabbing method. These results suggest that the tape-stripping method is comparable to the swabbing method for collecting viable skin bacteria, without losing fidelity to the composition of skin microbiome.

References

Jul 1, 1990·The Journal of Hospital Infection·A HambraeusW Whyte
Jan 1, 1989·The Journal of Hospital Infection·W WhyteA Hambraeus
Aug 1, 1967·Journal of the American Oil Chemists' Society·D M Updegraff
May 4, 2006·The Journal of Clinical Investigation·Julia A Segre
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Feb 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zhan GaoMartin J Blaser
Oct 31, 2007·Nursing·Jill Rushing
May 27, 2008·Genome Research·Elizabeth A GriceJulia A Segre
Sep 26, 2008·Diagnostic Microbiology and Infectious Disease·Kenneth G Van HornDenise Sebeck
May 30, 2009·Science·Elizabeth A GriceJulia A Segre
Nov 7, 2009·Science·Elizabeth K CostelloRob Knight
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Aug 13, 2010·Journal of Clinical Microbiology·Zhan GaoMartin J Blaser
Aug 17, 2010·Bioinformatics·Robert C Edgar
Mar 17, 2011·Nature Reviews. Microbiology·Elizabeth A Grice, Julia A Segre
Jun 24, 2011·The Journal of Investigative Dermatology·Kimberly A CaponeJaneta Nikolovski
Jul 16, 2011·Skin Pharmacology and Physiology·B Lange-AsschenfeldtJ Lademann
Feb 16, 2012·BMC Bioinformatics·Andre P MasellaJosh D Neufeld
Jun 16, 2012·Nature·Human Microbiome Project Consortium
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Jan 23, 2013·The Journal of Investigative Dermatology·Sorel Fitz-GibbonHuiying Li
Nov 19, 2013·Trends in Microbiology·Nina N Schommer, Richard L Gallo
Nov 26, 2013·Seminars in Immunology·James A Sanford, Richard L Gallo
Nov 22, 2014·Science·Yasmine Belkaid, Julia A Segre
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Jan 9, 2015·Clinical Microbiology Reviews·Jean-Christophe LagierDidier Raoult
Apr 16, 2015·PloS One·Ahmed BassiouniPeter-John Wormald
Jun 26, 2015·Science Translational Medicine·Dezhi KangHuiying Li
Dec 22, 2016·Scientific Reports·Emma BarnardHuiying Li

Citations

Nov 20, 2019·International Journal of Cosmetic Science·R SfrisoR Campiche
Jan 21, 2020·Drug Discoveries & Therapeutics·Nana C BenderliGabriel Loni Ekali
Jul 11, 2020·Biological Research for Nursing·Kana ShibataJunko Sugama
Nov 23, 2019·Scientific Reports·Rie Dybboe BjerreLars Engstrand
May 28, 2019·Microbiome·Ashley A RossJosh D Neufeld
Sep 29, 2020·Frontiers in Medicine·Satoshi NagaseShigefumi Okamoto
Dec 29, 2020·The British Journal of Dermatology·A J HughesR F L O'Shaughnessy
Mar 27, 2021·American Journal of Infection Control·Stéphanie F Bernatchez, Kimberly Schommer
May 19, 2021·Microbiome·Jacob T NearingMorgan G I Langille
May 31, 2021·Microbiome·Manon BoxbergerBernard La Scola

Datasets Mentioned

BETA
DRA006958

Methods Mentioned

BETA
Assay
PCR
amplicon sequencing
biopsy

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