A comparison of the accuracy of the corpuscular fragility and mean corpuscular volume tests for the alpha-thalassemia 1 and beta-thalassemia traits

International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics
Supatra SirichotiyakulTheera Tongsong

Abstract

To compare the accuracy of the osmotic fragility test (OFT) and mean corpuscular volume (MCV) calculation when screening for the alpha-thalassemia 1 and/or beta-thalassemia trait. In this cross-sectional study, blood samples from 328 apparently healthy pregnant women were sent on the same day to separate laboratories for the OFT (performed using a glycerol 0.45%, phosphate-buffered, sodium chloride solution) and MCV testing (by means of a standard automated hematology analyzer). A polymerase chain reaction was also performed to positively diagnose alpha-thalassemia 1 carriers, and a quantitative HbA(2) test to positively diagnose beta-thalassemia carriers. Sensitivity and specificity were 95.0% and 86% for the OFT; and based on the cut-off point of 78.1 fL derived from the ROC curve, they were 93% and 93.4% for MCV calculation. The latter test was found to be slightly more accurate than the OFT in predicting the presence of the alpha-thalassemia 1 and/or beta-thalassemia trait. Both tests have high screening sensitivity for the alpha-thalassemia 1 and/or beta-thalassemia traits, and their simplicity and very low cost make them attractive as screening tests for large populations. Since MCV seems to provide fewer false-positive r...Continue Reading

References

Apr 5, 2013·Prenatal Diagnosis·Theera TongsongSavitree Pranpanus
Feb 21, 2020·The Journal of Obstetrics and Gynaecology Research·Chanhathai NunchaiTheera Tongsong
Mar 16, 2011·The Tohoku Journal of Experimental Medicine·Zhanhui OuXiaofang Sun

Citations

Jan 1, 1987·Hemoglobin·Supan Fucharoen, P Winichagoon
Sep 1, 1996·Human Genetics·M Lemmens-ZygulskaG Flatz
Apr 17, 1998·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Chanane WanapirakA Tuggapichitti
May 27, 2005·The Journal of Obstetrics and Gynaecology Research·Supatra SirichotiyakulTheera Tongsong

Related Concepts

Prevalence Studies
Buffers
BSND gene
Osmotic Fragility Test
Prenatal Care brand of multivitamin
Hematology (Discipline)
Glycerin
Alpha-Thalassemia
Hematology Procedure
Pregnancy Complications, Hematologic

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