PMID: 8468453May 1, 1993Paper

A comparison of the detection sensitivity of lymphocyte membrane antigens using fluorescein and phosphor immunoconjugates

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
H B BeverlooHans J Tanke

Abstract

In this study we compared the sensitivity of immunocytochemical procedures, using conventional and time-resolved fluorescent dyes, in a model system consisting of paraformaldehyde-fixed human lymphocytes. The lymphocytes were stained for the presence of the CD4 epitope by indirect immunofluorescence using FITC as label or by using time-resolved luminescent immunophosphors. These immunophosphors were primarily developed for use under time-resolved fluorescence conditions, but they are also very well suited for use in conventional fluorescence microscopes. The differently labeled cells were first examined visually with a conventional fluorescence microscope in a double-blind study. The fluorescence was also measured with a CCD camera mounted on a specially constructed time-resolved fluorescence microscope which allows the suppression of the fast decaying fluorescence, thereby permitting visualization of the specific, slowing decaying luminescence of the phosphor label. With this microscope FITC and immunophosphor labeled lymphocytes were compared under normal conditions (i.e., continuous excitation) and under conditions of time-resolved registration. Conventional fluorescence microscopy revealed a better sensitivity in favor of t...Continue Reading

Citations

Jul 17, 1995·Biochimica Et Biophysica Acta·M Zoratti, I Szabò
Feb 27, 1999·Cellular Signalling·T ChakrabortiS Chakraborti
Jan 12, 2001·Analytical Biochemistry·J HamplD E Cooper

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