A comparison of the effects of exogenous and endogenous prostaglandins on fast and slow contractions of field-stimulated guinea-pig vas deferens

British Journal of Pharmacology
A M McKay, N L Poyser

Abstract

1. This study has compared the effects of exogenous and endogenous prostaglandins on the two phases of contraction of the guinea-pig vas deferens produced by electrical field stimulation. Prostaglandin E2 (PGE2), sulprostone and arachidonic acid dose-dependently and completely inhibited the first (fast) phase of contraction, with IC50s of 2.6 nM, 0.65 nM and 2.2 microM, respectively. 2. Following desensitization of the receptor for adenosine triphosphate (ATP) with alpha, beta-methylene ATP, PGE2, sulprostone and arachidonic acid dose-dependently inhibited the second (slow) phase of contraction of the guinea-pig vas deferens produced by electrical field stimulation, but the inhibition was incomplete (up to only 30%). Indomethacin (2.8 microM) reduced the effect of arachidonic acid. On its own, indomethacin (0.3 to 6.0 microM) had no consistent effect although, on some tissues, a slight potentiation of the contractions was seen. 3. Cicaprost (a PGI2 analogue) at low concentrations (0.5 to 30 nM) potentiated the first phase of contraction but even at high concentrations, had no consistent effect on the second phase of contraction of the guinea-pig vas deferens produced by electrical field stimulation. 4. PGE2, sulprostone and cic...Continue Reading

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