A comparison of the efficiency in serotyping of Pseudomonas aeruginosa from cystic fibrosis patients using monoclonal and polyclonal antibodies

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
B OjeniyiV T Rosdahl

Abstract

A well-known problem in serotyping Pseudomonas aeruginosa strains from cystic fibrosis patients using polyclonal sera is that more than half of the strains cannot be assigned to a single O-serogroup because of the high occurrence of polyagglutinable strains and nontypable strains. In the present study, the efficiency of a set of O-specific monoclonal antibodies in the simple slide agglutination test was compared with polyclonal sera for the serotyping of 243 isolates of P. aeruginosa from cystic fibrosis patients. Using the monoclonal antibodies, 213 (88%) strains were found to be typable and only 30 (12%) strains were nontypable. In contrast, when the polyclonal sera from Statens Seruminstitut were used, only 53 (22%) strains were typable. Similar results were obtained when polyclonal sera manufactured by Difco were used where only 61 (25%) strains were typable. We also investigated the consistency of each set of antibodies in typing of the same isolates on different days and found that the polyclonal sera showed higher reproducibility. The Statens Seruminstitut sera were found to have an 86% reproducibility while the Difco sera scored 81%; however, the percentage of strains that are nontypable remains below 25% despite the hi...Continue Reading

References

Feb 24, 1978·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·V Fiedlerová, J Davídek
Apr 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T L Pitt
Apr 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·B Ojeniyi
Mar 1, 1969·The Journal of Infectious Diseases·P V Liu

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Citations

May 1, 1990·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·B OjeniyiN Høiby
Feb 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·B OjeniyiV T Rosdahl
Jun 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·B OjeniyiV T Rosdahl
Feb 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·B OjeniyiN Høiby
Jun 7, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Lisa SaimanUNKNOWN Cystic Fibrosis Foundation

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