PMID: 9167270May 1, 1997Paper

A comparison of the germline potential of differently aged ES cell lines and their transfected descendants

Transgenic Research
L M FedorovJ Hirchenhain

Abstract

The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gene trap experiments were tested for their ability to colonize the germline. The maximal g.l.t. age for wild-type ES cells was equal to passage 26 and for transfected clones was equivalent to passage 32 of parental lines. It is shown that wild-type ES cells of less than a passage 15 should be used for effective production of transgenic g.l.t. clones. A simple system is outlined to evaluate the probability of g.l.t. on the basis of the chimaeric progeny obtained.

Citations

Oct 23, 2001·Genesis : the Journal of Genetics and Development·L M FedorovU R Rapp
Jun 18, 2005·The Journal of Biological Chemistry·Andreas WenzelJohannes von Lintig
Aug 10, 2012·Transgenic Research·Masumi HirabayashiShinichi Hochi
Feb 13, 2014·In Vitro Cellular & Developmental Biology. Animal·Anagha S BockElizabeth C Bryda
Oct 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Samuel M PopeMarc E Rothenberg
Sep 11, 2007·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Elizabeth D HughesThomas L Saunders
Dec 29, 2015·Histochemistry and Cell Biology·Qi TianLev M Fedorov
Dec 14, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Christopher M WardAnn Marie Flenniken
Jan 4, 2008·Physiological Genomics·Helen E MacLeanJeffrey D Zajac
Jan 30, 2004·Trends in Genetics : TIG·Eunju SeongMargit Burmeister

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