Jun 6, 2014

Visualization of the initial fixed nitrogen transport in nodulated soybean plant using [13N]N2 tracer gas

BioRxiv : the Preprint Server for Biology
Klaus M. StiefelDavid B. Joyce


The observation of initial transport of fixed nitrogen in intact soybean plants in real-time was conducted by using the positron-emitting tracer imaging system (PETIS). Soybean root nodules were fed with [13N]N2 for 10 minutes, and the radioactivity of [13N]N tracer was recorded for 60 minutes. The serial images of nitrogen fixation activity and translocation of fixed nitrogen in the soybean plant were reconstructed to estimate the fixed-N transport to the upper shoot. As a result, the signal of nitrogen radiotracer moving upward through the intact stem was successfully observed. This is the first report that the translocation of fixed-N is visualized in real-time in soybean plant by a moving image. The signal of nitrogen radiotracer appeared at the base stem at about 20 minutes after the feeding of tracer gas and it took 40 minutes to reach the upper stem. The velocity of fixed nitrogen translocation was estimated approximately at 1.63 cm min-1. The autoradiography taken after PETIS experiment showed a clear picture of transport of fixed 13N in the whole plant that the fixed-N moved not only via xylem system but also via the phloem system to the shoot after transferring from xylem to phloem in the stem although it has been gen...Continue Reading

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Apogon imberbis
Cell Growth
Shrimp allergenic extract
Anahita fauna

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
B David A NaafsAndy Ridgwell
Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine
Norbert KeutgenShoji Hashimoto
© 2020 Meta ULC. All rights reserved