PMID: 7013316Jan 1, 1980Paper

A comparison of the IFA and the ELISA for the demonstration of antibodies against schistosome gut-associated polysaccharide antigens in schistosomiasis

Zeitschrift Für Parasitenkunde
A M Deelder, D Kornelis

Abstract

The applicability of two immunodiagnostic techniques was studied for the detection of antibodies against schistosome gut-associated polysaccharide antigens in human schistosomiasis mansoni: the immunofluorescent antibody reaction (IFA) using Rossman's fixed paraffin sections of adult worms and the enzyme-linked immunosorbent assay (ELISA) with a trichloroacetic acid soluble fraction of total adult worm antigens (AWA-TCA). With the IFA, gut-associated polysaccharide antigens could be demonstrated with an anti-IgM conjugate in a high percentage of the sera tested, although false-negative reactions were occasionally recorded. The use of an anti-IgG conjugate resulted in the demonstration of antibodies against additional antigens in the parenchyma of the worm and on the tegument. Specific IgM antibodies were present in higher concentrations in the sera from children than in those from adults. Using AWA-TCA as the antigen preparation in the ELISA, only antibodies against the circulating anodic antigen (CAA) could be demonstrated. Pretreatment of the ELISA-plates with poly-L-lysine to couple AWA-TCA was not necessary. The ELISA was successfully applied with anti-Ig, anti IgG, and anti-IgM conjugates. With anti-Ig conjugate the test w...Continue Reading

References

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Citations

May 1, 1989·Transactions of the Royal Society of Tropical Medicine and Hygiene·A M DeelderW Duchenne
Jan 1, 2004·Trends in Parasitology·Michael J DoenhoffJoanne V Hamilton
Mar 4, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·Edward José de OliveiraMário Hiroyuki Hirata
Nov 26, 1999·Biochimica Et Biophysica Acta·R D Cummings, A K Nyame
Jul 16, 2002·Memórias do Instituto Oswaldo Cruz·Herminia Yohko KanamuraLuiz Candido de Souza Dias
Aug 23, 2005·Memórias do Instituto Oswaldo Cruz·Edward José de OliveiraDirce Mary Correia Lima
Apr 18, 2003·Cadernos de saúde pública·Edward José de OliveiraRicardo Mário de Carvalho Ciaravolho
Mar 1, 1996·Revista Da Sociedade Brasileira De Medicina Tropical·D M LimaS A Vellosa

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