PMID: 21932655Sep 22, 2011Paper

A comparison of the myogenic characteristics between stem cell antigen-(1+) and stem cell antigen-(1-) cells derived from skeletal muscle

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology
Xiaoxia CheJie Guo


To probe into the function of stem cell antigen-1 (Sca-1) in cell proliferation and differentiation. Sca-(1+) and Sca-(1-) muscle derived cells were separated from C57BU6 mice by fluorescence-activated cell sorting and then cultured in vitro. After 5 days cells proliferative curve were drawn according CCK-8 experimental results. Western blot also were done to detect Sca(-1), MyoD and Myogenin expression in cultured Sca(-1+) and Sca-(1-) muscle derived cells. The difference of the proliferative curve of Sca-(1+) and Sca-(1-) muscle derived cells cultured 3 days in vitro was not apparent, but Sca-(1-) muscle derived cells had a accelerated division rate in the follow days compared to the Sca-(1+) muscle derived cells. Sca-1 expression in both cells was not obvious. MyoD and Myogenin expression were stronger in Sca-(1+) than Sca-(1-) muscle derived cells. Sca-1 expression in muscle derived cells takes a period of time that related to the beginning and ending of the cell cycle.

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