PMID: 3748610Aug 1, 1986Paper

A comparison of the surface and secretions of Trichinella pseudospiralis and T. spiralis

Parasitology
N M AlmondR M Parkhouse

Abstract

Intact, viable adults, infective and newborn larvae of Trichinella pseudospiralis were surface labelled with 125I by the chloramine T method and labelled proteins were compared with those obtained from equivalent stages of T. spiralis. Electron-microscope autoradiography determined that labelled proteins were restricted to the cuticle for all stages of both isolates. Comparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE), using thin gradient gel slabs, of proteins obtained from each stage, demonstrated that the profile of surface-labelled proteins of T. pseudospiralis were restricted in number, stage specific, and similar to equivalent proteins of T. spiralis both in size and in their organization into aggregates. The stage-specific profiles of surface-labelled proteins derived from newborn larvae were indistinguishable, but differences were noted between adults and infective larvae of the two isolates. These differences in protein structure were confirmed by two dimensional mapping of tryptic peptides. Stage-specific profiles were also obtained when [35S]methionine biosynthetically labelled secretions of the 3 stages of T. pseudospiralis were compared by SDS-PAGE. Comparison of the...Continue Reading

References

Nov 1, 1986·Parasite Immunology·G Ortega-PierresR M Parkhouse
Jun 1, 1985·International Journal for Parasitology·C PalmasW Cabaj
Jan 1, 1974·Transactions of the Royal Society of Tropical Medicine and Hygiene·J AndersonT F de Marshall
Aug 1, 1984·Parasitology·R M Parkhouse, G Ortega-Pierres
Dec 1, 1983·Molecular and Biochemical Parasitology·R M Parkhouse, N W Clark
Jan 1, 1981·Parasite Immunology·R M ParkhouseB M Ogilvie
Oct 9, 1980·Nature·M PhilippB M Ogilvie
Jan 1, 1963·Transactions of the Royal Society of Tropical Medicine and Hygiene·F H BUDDEN

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Citations

Jun 1, 1985·International Journal for Parasitology·C PalmasW Cabaj
May 1, 1996·International Journal for Parasitology·D Wakelin, P K Goyal
Jun 1, 1987·Molecular and Biochemical Parasitology·F QureshiH R Miller
Jun 1, 1990·Molecular and Biochemical Parasitology·A M GoldS W Buck
Jun 23, 2001·Molecular and Biochemical Parasitology·S KuratliB Connolly
Jun 1, 1996·Molecular and Biochemical Parasitology·D K VassilatisL H Van der Ploeg
Jan 1, 1989·Parasitology·R M Parkhouse, L J Harrison
Jun 1, 1990·Parasitology·F Bolas-Fernandez, D Wakelin
Jun 1, 1993·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·F Bolas-FernandezA R Martinez-Fernandez
May 21, 2003·Journal of Helminthology·F Bolas-Fernández

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