A comparison of the surface nanostructure from two different types of gram-negative cells: Escherichia coli and Rhodobacter sphaeroides

Micron : the International Research and Review Journal for Microscopy
Zachery OestreicherYoshihiro Fukumori

Abstract

Bacteria have been studied using different microscopy methods for many years. Recently, the developments of high-speed atomic force microscopy have opened the doors to study bacteria in new ways due to the fact that it uses much less force on the sample while imaging. This makes the high-speed atomic force microscope an indispensable technique for imaging the surface of living bacterial cells because it allows for the high-resolution visualization of surface proteins in their natural condition without disrupting the cell or the activity of the proteins. Previous work examining living cells of Magnetospirillum magneticum AMB-1 demonstrated that the surface of these bacteria was covered with a net-like structure that is mainly composed of porin molecules. However, it was unclear whether or not this feature was unique to other living bacteria. In this study we used the high-speed atomic force microscope to examine the surface of living cells of Escherichia coli and Rhodobacter sphaeroides to compare their structure with that of M. magneticum. Our research clearly demonstrated that both of these types of cells have an outer surface that is covered in a network of nanometer-sized holes similar to M. magneticum. The diameter of the h...Continue Reading

References

Sep 5, 1988·Journal of Molecular Biology·M KesselA C Steven
Jan 19, 1987·FEBS Letters·J P ChalcroftW Baumeister
Jan 1, 1984·Annual Review of Biochemistry·D Eisenberg
Jan 1, 1996·Microbiology and Immunology·K AmakoA Takade
Jun 6, 1998·Journal of Structural Biology·T Schirmer
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·T AndoA Toda
Nov 1, 2002·Biochimica Et Biophysica Acta·Georg E Schulz
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido
Dec 25, 2007·Pflügers Archiv : European journal of physiology·Toshio AndoHayato Yamashita
Aug 1, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Vincent DupresYves F Dufrêne
Oct 22, 2009·Molecular Microbiology·Szymon JarosławskiSimon Scheuring
May 23, 2012·Journal of Molecular Biology·Hayato YamashitaYoshihiro Fukumori
Jul 10, 2012·Nature Nanotechnology·Ignacio CasusoSimon Scheuring
Jan 8, 2013·Microscopy·Toshio Ando
Jan 31, 2014·Chemical Reviews·Toshio AndoSimon Scheuring

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Citations

Mar 28, 2018·Nanoscale·Martyna MichalskaPhilip D Laible
Dec 20, 2017·Biophysics Reviews·Toshio Ando
Oct 18, 2019·The Analyst·Georgina BennBart W Hoogenboom
Oct 31, 2021·Proceedings of the National Academy of Sciences of the United States of America·Georgina BennBart W Hoogenboom

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