A comparison of the transcriptomes between diploid and autotetraploid Paulownia fortunei under salt stress

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Zhe WangHeping Cao

Abstract

Paulownia is a tree species grown in many countries. Our previous study reveals that tetraploid Paulownia fortunei is more tolerant to salt stress than its corresponding diploid tree. To investigate the molecular mechanisms of salt stress tolerance in P. fortunei, the transcriptomes of normal and salt-stressed diploid and tetraploid were investigated. After assembling the clean reads, we obtained 130,842 unigenes. The unigenes were aligned against six public databases (Nr, Nt, Swiss-Prot, COG, KEGG, GO) to discover homologs and assign functional annotations. We retrieved 7983 and 15,503 differentially expressed unigenes (DEUs) between the normal and the salt-stressed diploid and tetraploid P. fortunei, respectively. We identified dozens of important DEUs including 3 related to photosynthesis, 10 related to plant growth and development and 11 related to osmolytes. Some of these DEUs were upregulated in tetraploid compared to diploid and others were upregulated under salt stress. Quantitative reverse transcriptase polymerase chain reaction verified the expression patterns of 15 unigenes. Our results provided insights into the molecular aspects why tetraploid is stronger and more energetic than diploid under saline environment. Th...Continue Reading

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Citations

Dec 9, 2020·Plant Science : an International Journal of Experimental Plant Biology·Jinhe WangMin Li

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Datasets Mentioned

BETA
SRP060682

Methods Mentioned

BETA
transgenic
salt treatments
PCR
glycosylation
salt treatment
Illumina sequencing
RNA-seq
salt stress

Software Mentioned

DynamicTrim
Dynamic Trim
BLASTX
Beacon Designer
Solexa QA
LengthSort
Trinity
BLASTN
ESTScan
Blast2GO

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