A Comparison of Traditional and Novel Methods for the Separation of Exosomes from Human Samples

BioMed Research International
Li-Li YuMing-Bing Xiao

Abstract

Exosomes are discrete populations of small (40-200 nm in diameter) membranous vesicles that are released into the extracellular space by most cell types, eventually accumulating in the circulation. As molecular messengers, exosomes exert a broad array of vital physiologic functions by transporting information between different cell types. Because of these functional properties, they may have potential as biomarker sources for prognostic and diagnostic disease. Recent research has found that exosomes have potential to be utilized as drug delivery agents for therapeutic targets. However, basic researches on exosomes and researches on their therapeutic potential both require the existence of effective and rapid methods for their separation from human samples. In the current absence of a standardized method, there are several methods available for the separation of exosomes, but very few studies have previously compared the efficiency and suitability of these different methods. This review summarized and compared the available traditional and novel methods for the extraction of exosomes from human samples and considered their advantages and disadvantages for use in clinical laboratories and point-of-care settings.

References

Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Aug 5, 2008·Journal of Immunological Methods·Réjean CantinCaroline Gilbert
Sep 11, 2012·Biotechnology Advances·Ruenn Chai LaiSai Kiang Lim
Jun 11, 2014·World Journal of Gastroenterology : WJG·Lin ZhuXiao-Hang Zhao
Nov 15, 2014·Journal of Extracellular Vesicles·Dennis K JeppesenMarie S Ostenfeld
Apr 23, 2015·International Journal of Molecular Medicine·Rafal SzatanekMonika Baj-Krzyworzeka
May 20, 2015·Nature Cell Biology·Bruno Costa-SilvaDavid Lyden
Aug 21, 2015·Expert Review of Molecular Diagnostics·Theresa L Whiteside
Jan 19, 2016·Journal of Visualized Experiments : JoVE·Kateena Addae KonaduVincent Bond
Mar 4, 2017·Theranostics·Pin LiZhiqiang Gao
Oct 31, 2017·Nature Reviews. Nephrology·Michael L MerchantJon B Klein
Nov 2, 2017·ACS Nano·Fei LiuUtkan Demirci

❮ Previous
Next ❯

Citations

Jan 26, 2020·International Journal of Molecular Sciences·Hyeon Su JooJung Min Lee
Jan 17, 2020·The Journal of Physiology·Ivan J VechettiJohn J McCarthy
Mar 5, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Akhil SrivastavaRajagopal Ramesh
Mar 30, 2019·Translational Psychiatry·Saumeh SaeediGustavo Turecki
Jul 25, 2019·International Journal of Molecular Sciences·Aneta ZebrowskaMonika Pietrowska
Jul 10, 2020·Skeletal Muscle·Laura Le GallStephanie Duguez
Sep 13, 2020·Cancers·Mariantonia LogozziStefano Fais
Aug 14, 2019·International Journal of Molecular Sciences·Parkyong SongJong Hyuk Yoon
Jun 4, 2020·Cancer Cell International·Leyla Norouzi-BaroughSadegh Shirian
Sep 10, 2020·International Journal of Molecular Sciences·Karim SidhomAyesha Saleem
Feb 8, 2020·Cellular and Molecular Bioengineering·Zhenjiang ZhangMichael R King
Jun 18, 2020·Frontiers in Molecular Neuroscience·Zhiqi SongChuan Qin
Jul 9, 2020·Scientific Reports·Elena Martínez-GonzálezAmanda Fernández-Rodríguez
Dec 2, 2020·Pharmaceutics·Laia Hernandez-OllerSimo Schwartz
Jan 21, 2021·Regenerative Medicine·Antara BanerjeeSurajit Pathak
Oct 22, 2020·Biotechnology and Bioengineering·Sara Abdollahi
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Isha GauravZhijun Yang
Apr 7, 2021·The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry·Mohini RanganathanThalachallour Mohanakumar
May 1, 2021·International Journal of Molecular Sciences·Małgorzata S Sabina MałysRenate Kain
Apr 30, 2021·Cell Biology International·Ghadi N AlzhraniNehal Elsherbiny
May 19, 2021·Translational Vision Science & Technology·Hanan ElShelmaniDavid Keegan
Jul 3, 2021·International Journal of Molecular Sciences·Lucy BirtwistleCarol Pollock
Sep 26, 2020·Current Cancer Drug Targets·Prasad PofaliVaishali Londhe
May 8, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Guanghong Jia, James R Sowers
Aug 10, 2021·Journal of Biotechnology·Julian C KrappingerJulia Feichtinger
Oct 14, 2019·ACS Biomaterials Science & Engineering·Yanyan LiNianping Feng
Jan 19, 2021·ACS Biomaterials Science & Engineering·Camilo Mora-NavarroDonald O Freytes
Aug 28, 2021·Biomedicines·Maria I Mosquera-HerediaPilar Garavito-Galofre
Jun 11, 2020·Current Molecular Medicine·Mohammadbagher HosseiniZakiyeh Ebadi

❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient zone centrifugation
density gradient centrifugation
Precipitation
enzyme-linked immunosorbent assay
chip
bronchoalveolar lavage

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Pharmaceutics
Xin-Chi Jiang, Jian-Qing Gao
Journal of Nanobiotechnology
Edwin J BunggulawaGuixue Wang
International Journal of Clinical and Experimental Medicine
Zhen QuYitao Ding
© 2022 Meta ULC. All rights reserved