A comparison of transcriptomic patterns measured in the skin of Chinese fine and coarse wool sheep breeds

Scientific Reports
Lichun ZhangAaron B Ingham

Abstract

We characterised wool traits, and skin gene expression profiles of fine wool Super Merino (SM) and coarse wool Small Tail Han (STH) sheep. SM sheep had a significantly higher total density of wool follicles, heavier fleeces, finer fibre diameter, and increased crimp frequency, staple length and wool grease (lanolin) production. We found 435 genes were expressed at significantly different levels in the skin of the two breeds (127 genes more highly in SM and 308 genes more highly in STH sheep). Classification of the genes more highly expressed in SM sheep revealed numerous lipid metabolic genes as well as genes encoding keratins, keratin-associated proteins, and wool follicle stem cell markers. In contrast, mammalian epidermal development complex genes and other genes associated with skin cornification and muscle function were more highly expressed in STH sheep. Genes identified in this study may be further evaluated for inclusion in breeding programs, or as targets for therapeutic or genetic interventions, aimed at altering wool quality or yield. Expression of the lipid metabolic genes in the skin of sheep may be used as a novel trait with the potential to alter the content or properties of lanolin or the fleece.

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Methods Mentioned

BETA
PCR
ELISA
GTPase
light microscopy
cDNA library construction
RNA-seq

Software Mentioned

Cytoscape
Ingenuity Pathway Analysis ( IPA )
Excel
EnrichR
FastQC Genomics Workbench
GeneMANIA
IPA

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