A comparison of two histological validating techniques for occlusal caries

Journal of Dentistry
D N RickettsE A Kidd

Abstract

Validation of a diagnostic technique is important to establish whether it actually measures what it is purported to measure. However, the accuracy of the validation technique per se can influence the apparent accuracy of the diagnostic technique. The aim of this study was to describe two alternative histological validating techniques for occlusal caries and to compare quantitative depth measurements of carious lesions taken using each method. Thirty sections (mean thickness 0.67 mm) were cut to include two to four discrete sites in 10 freshly extracted teeth. The first histological validating technique used a microfocal X-ray unit to produce magnified high definition radiographic images of the sections or 'macroradiographs'. An image analysis system was used to make quantitative measurements of the lesions (if present) with respect to the enamel-dentine junction (EDJ). The second validating technique used a confocal microscope to image beneath the cut surface of the section. Quantitative measurements were taken from the fluorescence images of both sides of each section and a mean depth measurement calculated. Complete agreement was found between the two validating techniques for the subjective interpretation of the presence and...Continue Reading

References

Jan 1, 1992·Caries Research·C PenningJ M ten Cate
Mar 1, 1989·The British Journal of Radiology·J C Buckland-Wright, C R Bradshaw
Mar 1, 1989·The British Journal of Radiology·J C Buckland-Wright
Aug 1, 1983·Scandinavian Journal of Dental Research·M Juhl
Aug 1, 1961·Acta Odontologica Scandinavica·G GUSTAFSON, A G GUSTAFSON

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Citations

Mar 28, 2012·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·M D M OliveiraR O Rocha
Mar 12, 2010·Lasers in Medical Science·Anahita Jablonski-MomeniKlaus Pieper
Sep 29, 2009·Lasers in Medical Science·Andréia Bolzan de PaulaJonas Almeida Rodrigues
Feb 3, 2016·Journal of Dentistry·Mahdi Shahmoradi, Michael Swain
Aug 3, 2004·Journal of Dental Research·M-C D N J M Huysmans, C Longbottom

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