A comparison of two West Nile virus detection assays (TaqMan reverse transcriptase polymerase chain reaction and VecTest antigen assay) during three consecutive outbreaks in northern Illinois

Journal of the American Mosquito Control Association
Richard L LampmanRobert J Novak

Abstract

Mosquitoes identified as female Culex (Culex) species, primarily mixtures or uniform batches of Culex pipiens and Culex restuans, were collected daily from gravid traps by 2 mosquito abatement districts (MADs) in Cook County, Illinois. From 2002 through 2004, batches (pools) of mosquitoes were tested by the MADs for West Nile virus (WNV) by using VecTest WNV antigen assays and the same samples were retested, usually within 1-2 wk, for WNV RNA by the TaqMan reverse transcriptase polymerase chain reaction (RT-PCR). There were 952 TaqMan-positive pools out of 3,953 pools over the 3 years, and about one half of that number were VecTest-positive. The difference between the 2 detection assays varied between and within years. The VecTest assays detected about 57% and 69% of the TaqMan RT-PCR-positive pools from Des Plaines Valley MAD and Northwest MAD in 2002, but only about 40% and 46% in 2003, and 36% and 55% in 2004, respectively. Based on a subset of the 2004 data, a linear relationship was found between VecTest detection of WNV and TaqMan cycle threshold between 18 and 28 cycles. A temporal decrease in the difference between the 2 assays was observed in 2003 and 2004, which we conjecture is due, at least partially, to a seasonal ...Continue Reading

References

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Citations

May 10, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·Weidong GuRobert J Novak
Apr 22, 2014·Vector Borne and Zoonotic Diseases·Allison M GardnerEphantus J Muturi
Dec 5, 2008·Preventive Veterinary Medicine·Michael P Ward
Oct 28, 2006·Journal of the American Mosquito Control Association·Richard LampmanRobert Novak
Feb 5, 2008·Journal of the American Mosquito Control Association·Genevieve L Sutherland, Roger S Nasci
Nov 17, 2019·International Journal of Environmental Research and Public Health·Paul T LeisnhamShannon L LaDeau
Aug 1, 2008·Journal of the American Mosquito Control Association·Daniel M ChisenhallChristopher N Mores
Aug 8, 2013·Journal of the American Mosquito Control Association·Richard L LampmanRobert J Novak
Sep 17, 2021·Virology Journal·Tomo DaidojiTakaaki Nakaya

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Methods Mentioned

BETA
Assay

Software Mentioned

SPSS
TaqMan
VecTest

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