A competitive ELISA for the specific diagnosis of contagious bovine pleuropneumonia (CBPP)

Veterinary Microbiology
C Le Goff, F Thiaucourt

Abstract

A competitive ELISA, using a specific monoclonal antibody, was designed to detect antibodies to Mycoplasma mycoides subsp. mycoides SC, the agent of contagious bovine pleuropneumonia. One monoclonal antibody was found suitable for such a test, '117/5', it does not cross-react with any of the other mycoplasma species tested, furthermore, its binding is inhibited by positive sera. The cutoff, 50% of inhibition, was determined using a set of negative sera from CBPP-free areas. The sensitivity was controlled with sera from artificially infected animals as well as from sera from areas where CBPP is enzootic. In both cases, cELISA compared favorably with CFT. The precocity of detection was similar but cELISA detected more positives and the positive titers seemed to persist longer than in the case of CFT. Lysis of the antigen used to coat the ELISA plates reduced the variability of fixation and improved the repeatability of the test. A field evaluation is now in progress which will determine the true sensitivity and specificity of the test and also check if antibodies are detected after vaccination.

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Citations

Jul 9, 2013·Bulletin de la Société de pathologie exotique·M MbengueY Thiongane
Oct 18, 2002·Veterinary Microbiology·José M Martin AlonsoFrancisco Parra
Feb 18, 2011·BMC Genomics·Francois ThiaucourtPascal Sirand-Pugnet
Aug 9, 2011·Journal of Bioscience and Bioengineering·Yusuke MiyachiAkihiko Kondo
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Jun 29, 2004·Preventive Veterinary Medicine·Matthieu LesnoffFrançois Thiaucourt
Jun 2, 2016·The Onderstepoort Journal of Veterinary Research·Garuma DanielBenti Deresa
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May 12, 2020·Veterinary Pathology·Giovanni Di TeodoroMassimo Scacchia
Feb 24, 2001·Annals of the New York Academy of Sciences·F ThiaucourtA Provost
Oct 30, 2018·Frontiers in Veterinary Science·Mahamadou Seyni YansambouRianatou Bada Alambedji
Mar 18, 2003·The Veterinary Record·B CetinkayaF Thiaucourt
Aug 8, 2021·Animals : an Open Access Journal From MDPI·Elizabeth A ClemmonsJohn W Dutton
May 31, 2011·Preventive Veterinary Medicine·Florence TardyFrançois Poumarat

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