A competitive enzyme immunoassay for the quantitative detection of cocaine from banknotes and latent fingermarks

Forensic Science International : Synergy
Susan van der HeideDavid A Russell

Abstract

A sensitive and versatile competitive enzyme immunoassay (cEIA) has been developed for the quantitative detection of cocaine in complex forensic samples. Polyclonal anti-cocaine antibody was purified from serum and deposited onto microtiter plates. The concentration of the cocaine antibody adsorbed onto the plates, and the dilution of the cocaine-HRP hapten were both studied to achieve an optimised immunoassay. The method was successfully used to quantify cocaine in extracts taken from both paper currency and latent fingermarks. The limit of detection (LOD) of 0.162ngmL(-1) achieved with the assay compares favourably to that of conventional chromatography-mass spectroscopy techniques, with an appropriate sensitivity for the quantification of cocaine at the low concentrations present in some forensic samples. The cEIA was directly compared to LC-MS for the analysis of ten UK banknote samples. The results obtained from both techniques were statistically similar, suggesting that the immunoassay was unaffected by cross-reactivity with potentially interfering compounds. The cEIA was used also for the detection of cocaine in extracts from latent fingermarks. The results obtained were compared to the cocaine concentrations detected in...Continue Reading

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Citations

Mar 16, 2016·Science & Justice : Journal of the Forensic Science Society·Annemieke van DamSaskia A G Lambrechts
Mar 24, 2016·Journal of Pharmaceutical and Biomedical Analysis·Juan C VidalJuan R Castillo
May 18, 2016·Analytica Chimica Acta·L S Castillo-Peinado, M D Luque de Castro
Jun 24, 2016·Drug Testing and Analysis·Emine GulerHakan Coskunol
Aug 17, 2016·The Analyst·Susan van der HeideDavid A Russell
Mar 17, 2020·Journal of Forensic Sciences·Marina GonzálezRenata Pereira Limberger
Dec 3, 2020·Journal of Analytical Toxicology·Caroline PollardKim Wolff
Sep 7, 2020·Forensic Science International : Synergy·Paul BowdlerKevin C Honeychurch

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