A competitive enzyme-linked immunosorbent assay (ELISA) to detect retronecine and monocrotaline in vitro

Toxicon : Official Journal of the International Society on Toxinology
M A BoberM J Kurth

Abstract

Antibodies to the nonesterified pyrrolizidine nucleus, retronecine (155 mol.wt), were produced in rabbits and detected using an avidin-biotin antibody ELISA. A competitive ELISA for the detection of retronecine and the cyclic diester monocrotaline was also developed using the antiserum produced against the hapten conjugate, retronecine-bovine serum albumin. Retronecine was obtained by hydrolysis of monocrotaline, succinylated and directly coupled to bovine serum albumin or ovalbumin. Antibodies to the pyrrolizidine nucleus, retronecine, can be detected within 5 min after the addition of substrate using the avidin-biotin ELISA. Competitive inhibition of antibodies to retronecine is obtained by the addition of known amounts (0-11.42 micrograms/microliters) of either the homologous antigen, retronecine, or the heterologous antigen, monocrotaline, however, retronecine acts as the better competitor.

References

Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B N AmesF D Lee
Feb 24, 1968·Nature·A R Mattocks
Jan 1, 1980·Perspectives in Biology and Medicine·R J Huxtable

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Citations

Sep 19, 2009·Analytical and Bioanalytical Chemistry·Colin CrewsRudi Krska
Jan 1, 1991·Critical Reviews in Food Science and Nutrition·U SamarajeewaM R Marshall
Dec 12, 2013·Analytical and Bioanalytical Chemistry·Michalina OplatowskaKatrina Campbell
Jan 22, 2002·Therapeutic Drug Monitoring·M J Stewart, V Steenkamp
May 15, 2003·Journal of Agricultural and Food Chemistry·Stephen T LeeBryan L Stegelmeier
Oct 29, 2000·Journal of Agricultural and Food Chemistry·S T LeeB L Stegelmeier

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