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A complete bacterial genome assembled de novo using only nanopore sequencing data

Nature Methods

Aug 1, 2015

Nicholas James LomanJared T Simpson

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Abstract

We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-Mb contig using only nanopore data. Our method has three stages: (i) overlaps are detected between reads and then corrected by a multiple-alignment process; (ii) corrected reads are assembled using the...read more

Mentioned in this Paper

Chromosomes
Computer Programs and Programming
Escherichia coli
Genome
Nucleotides
Reproducibility of Results
Surgical Flaps
Genome, Bacterial
Escherichia coli K12
Contig Mapping
170
6
2
942
2
Paper Details
References
    • References16
    • Citations69
    • References16
    • Citations69
  • A complete bacterial genome assembled de novo using only nanopore sequencing data

    Nature Methods

    Aug 1, 2015

    Nicholas James LomanJared T Simpson

    PMID: 26076426

    DOI: 10.1038/nmeth.3444

    Abstract

    We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-Mb contig using only nanopore data. Our method has three stages: (i) overlaps are detected between reads and then corrected by a multiple-alignment process; (ii) corrected reads are assembled using the...read more

    Mentioned in this Paper

    Chromosomes
    Computer Programs and Programming
    Escherichia coli
    Genome
    Nucleotides
    170
    6
    2
    942
    2

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    Paper Details
    References
    • References16
    • Citations69
    • References16
    • Citations69
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