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A complete bacterial genome assembled de novo using only nanopore sequencing data

Nature Methods

Jun 16, 2015

Nicholas J LomanJared T Simpson

Abstract

We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-Mb contig using only nanopore data. Our method has three stages: (i) overlaps are detected between reads and then corrected by a multiple-alignment process; (ii) corrected reads are assembled using the...read more

Mentioned in this Paper

Genome
SECTM1 protein, human
Escherichia coli K12
Nucleic Acid Sequencing
Surgical Flaps
Computer Programs and Programming
Sequencing
Sequence Determinations, DNA
Nucleotides
Chromosomes
169
6
2
897
2
Paper Details
References
  • References17
  • Citations126
12
  • References17
  • Citations126
12345...

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A complete bacterial genome assembled de novo using only nanopore sequencing data

Nature Methods

Jun 16, 2015

Nicholas J LomanJared T Simpson

PMID: 26076426

DOI: 10.1038/nmeth.3444

Abstract

We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-Mb contig using only nanopore data. Our method has three stages: (i) overlaps are detected between reads and then corrected by a multiple-alignment process; (ii) corrected reads are assembled using the...read more

Mentioned in this Paper

Genome
SECTM1 protein, human
Escherichia coli K12
Nucleic Acid Sequencing
Surgical Flaps
Computer Programs and Programming
Sequencing
Sequence Determinations, DNA
Nucleotides
Chromosomes
169
6
2
897
2

Similar Papers Found In These Feeds

Structural Biology: Biophysics

Structural Biology: Biophysics describes the study of structural biology using various biophysical techiniques such as imaging, computational modeling, etc. Discover the latest research on structrual biology here.

Nanopore Sequencing

Nanopore sequencing is a third generation pproach used in the sequencing of biopolymers- specifically, polynucleotides in the form of DNA or RNA. Discover the latest research on nanopore sequencing.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America

Replication of the escherichia coli K12 chromosome

Proceedings of the National Academy of Sciences of the United States of AmericaNovember 1, 1967
M Abe, J Tomizawa
Molecular & General Genetics : MGG

De novo gene duplication versus reactivation of cryptic genes in Escherichia coli K-12

Molecular & General Genetics : MGGJanuary 1, 1970
R CuninN Glansdorff
Paper Details
References
  • References17
  • Citations126
12
  • References17
  • Citations126
12345...
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