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A complete bacterial genome assembled de novo using only nanopore sequencing data

bioRxiv

Feb 20, 2015

Nicholas James LomanJared T Simpson

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Abstract

A method for de novo assembly of data from the Oxford Nanopore MinION instrument is presented which is able to reconstruct the sequence of an entire bacterial chromosome in a single contig. Initially, overlaps between nanopore reads are detected. Reads are then subjected to one or more ...read more

Mentioned in this Paper

Base Sequence
Chromosomes
Chromosomes, Bacterial
Escherichia coli
Genes
Genome
Nucleotides
Genome, Bacterial
Instrument - Device
Reconstructive Surgical Procedures
209
4
721
Paper Details
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  • A complete bacterial genome assembled de novo using only nanopore sequencing data

    bioRxiv

    Feb 20, 2015

    Nicholas James LomanJared T Simpson

    PMID: 990015552

    DOI: 10.1101/015552

    Abstract

    A method for de novo assembly of data from the Oxford Nanopore MinION instrument is presented which is able to reconstruct the sequence of an entire bacterial chromosome in a single contig. Initially, overlaps between nanopore reads are detected. Reads are then subjected to one or more ...read more

    Mentioned in this Paper

    Base Sequence
    Chromosomes
    Chromosomes, Bacterial
    Escherichia coli
    Genes
    209
    4
    721
    Paper Details
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