A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster.

Cell
Zhihao ZhengDavi D Bock

Abstract

Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. We developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly at synaptic resolution. To validate the dataset, we traced brain-spanning circuitry involving the mushroom body (MB), which has been extensively studied for its role in learning. All inputs to Kenyon cells (KCs), the intrinsic neurons of the MB, were mapped, revealing a previously unknown cell type, postsynaptic partners of KC dendrites, and unexpected clustering of olfactory projection neurons. These reconstructions show that this freely available EM volume supports mapping of brain-spanning circuits, which will significantly accelerate Drosophila neuroscience. VIDEO ABSTRACT.

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Methods Mentioned

BETA
dissection
electron microscopy
X-ray
light microscopy

Software Mentioned

MultiColor FlpOut
R
MB
BigWarp Fiji
CMTK
Docker Community Edition
CATMAID
MultiColor
TEMCA2
R NeuroAnatomy Toolbox

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