PMID: 12770825May 29, 2003Paper

A complete gene cluster from Streptomyces nanchangensis NS3226 encoding biosynthesis of the polyether ionophore nanchangmycin

Chemistry & Biology
Yuhui SunZixin Deng

Abstract

The PKS genes for biosynthesis of the polyether nanchangmycin are organized to encode two sets of proteins (six and seven ORFs, respectively), but are separated by independent ORFs that encode an epimerase, epoxidase, and epoxide hydrolase, and, notably, an independent ACP. One of the PKS modules lacks a corresponding ACP. We propose that the process of oxidative cyclization to form the polyether structure occurs when the polyketide chain is still anchored on the independent ACP before release. 4-O-methyl-L-rhodinose biosynthesis and its transglycosylation involve four putative genes, and regulation of nanchangmycin biosynthesis seems to involve activation as well as repression. In-frame deletion of a KR6 domain generated the nanchangmycin aglycone with loss of 4-O-methyl-L-rhodinose and antibacterial activity, in agreement with the assignments of the PKS domains catalyzing specific biosynthetic steps.

References

Jan 1, 1990·Methods in Enzymology·W R Pearson
Dec 3, 1971·Biochemical and Biophysical Research Communications·L K SteinraufM Pinkerton
Dec 3, 1971·Biochemical and Biophysical Research Communications·E W Czerwinski, L K Steinrauf
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Feb 21, 1995·Biochimica Et Biophysica Acta·K KosugaT Ono
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·S A Chervitz, J J Falke
Jun 1, 1997·Applied and Environmental Microbiology·M O'Connell-MotherwayD van Sinderen
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 16, 1998·Genome Research·D GordonP Green
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y XueD H Sherman
Dec 16, 1998·Trends in Biochemical Sciences·S F Altschul, E V Koonin
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·H IkedaS Omura
Nov 27, 1999·Drug Metabolism Reviews·A Radominska-PandyaP I Mackenzie
Apr 11, 2001·Trends in Biochemical Sciences·J J Falke, G L Hazelbauer
Jul 31, 2001·Natural Product Reports·B J Rawlings
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·S OmuraM Hattori
Jan 5, 2002·Journal of Industrial Microbiology & Biotechnology·P F LeadlayC J Wilkinson
Feb 20, 2002·Chemical Reviews·David A. Hopwood

❮ Previous
Next ❯

Citations

Mar 6, 2007·Journal of Molecular Evolution·Dongmei Liang, Jianjun Qiao
May 7, 2010·Journal of Industrial Microbiology & Biotechnology·Eric Cundliffe, Arnold L Demain
Apr 2, 2005·Current Opinion in Microbiology·Mervyn J Bibb
Feb 5, 2004·Phytochemistry·Tomás RezankaValery M Dembitsky
Oct 25, 2011·Chemical Reviews·Christopher J ThibodeauxHung-wen Liu
Feb 4, 2010·Journal of the American Chemical Society·Christopher T Walsh, Michael A Fischbach
Mar 23, 2011·Applied and Environmental Microbiology·Hao WangYing Huang
Dec 14, 2011·Applied and Environmental Microbiology·Chunyan JiangLinquan Bai
Jun 29, 2010·Journal of Bacteriology·Xiang-Jing WangWen-Sheng Xiang
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Gang LiuHuarong Tan
Jan 9, 2009·Bioscience, Biotechnology, and Biochemistry·Akira MigitaHideaki Oikawa
Apr 4, 2006·Trends in Microbiology·Kirsty J McLeanAndrew W Munro
Jun 26, 2013·Current Opinion in Chemical Biology·Atsushi MinamiHideaki Oikawa
Apr 25, 2006·Chemistry & Biology·Andrew R GallimoreJonathan B Spencer
Dec 13, 2016·Chemical Reviews·Man-Cheng TangYi Tang
Feb 6, 2017·Journal of the American Chemical Society·Xinqiang XieDavid E Cane
Apr 11, 2017·Chemical Reviews·Adrian T Keatinge-Clay
Jul 3, 2009·Angewandte Chemie·Ivan Vilotijevic, Timothy F Jamison
Mar 5, 2015·Chemical Society Reviews·Sherif I ElshahawiJon S Thorson
Feb 10, 2018·Journal of Industrial Microbiology & Biotechnology·Satoshi YuzawaLeonard Katz
Dec 7, 2013·Angewandte Chemie·Petra PöplauJörn Piel
Jul 25, 2018·Chembiochem : a European Journal of Chemical Biology·Song MengHai-Xue Pan
Apr 12, 2008·Chembiochem : a European Journal of Chemical Biology·Yuliya DemydchukPeter F Leadlay
Dec 6, 2008·Angewandte Chemie·Christopher J ThibodeauxHung-wen Liu
Nov 26, 2008·Chembiochem : a European Journal of Chemical Biology·Luke SmithPeter F Leadlay

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.