A complete picture of protein unfolding and refolding in surfactants.

Chemical Science
Jannik Nedergaard PedersenJan Skov Pedersen

Abstract

Interactions between proteins and surfactants are of relevance in many applications including food, washing powder formulations, and drug formulation. The anionic surfactant sodium dodecyl sulfate (SDS) is known to unfold globular proteins, while the non-ionic surfactant octaethyleneglycol monododecyl ether (C12E8) can be used to refold proteins from their SDS-denatured state. While unfolding have been studied in detail at the protein level, a complete picture of the interplay between protein and surfactant in these processes is lacking. This gap in our knowledge is addressed in the current work, using the β-sheet-rich globular protein β-lactoglobulin (bLG). We combined stopped-flow time-resolved SAXS, fluorescence, and circular dichroism, respectively, to provide an unprecedented in-depth picture of the different steps involved in both protein unfolding and refolding in the presence of SDS and C12E8. During unfolding, core-shell bLG-SDS complexes were formed within ∼10 ms. This involved an initial rapid process where protein and SDS formed aggregates, followed by two slower processes, where the complexes first disaggregated into single protein structures situated asymmetrically on the SDS micelles, followed by isotropic redist...Continue Reading

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Citations

Jul 28, 2020·Pharmaceutics·Ioannis AnestopoulosMihalis I Panayiotidis
Aug 6, 2020·Frontiers in Molecular Biosciences·Grethe Vestergaard JensenJan Skov Pedersen
Sep 10, 2020·International Journal of Environmental Research and Public Health·Luiz Alberto ColnagoCintia Hiromi Okino
Mar 7, 2021·International Journal of Molecular Sciences·Georgia-Persephoni VoulgaridouAglaia Pappa
Apr 13, 2021·Journal of Colloid and Interface Science·Henriette Gavlshøj MortensenJan Skov Pedersen
Aug 21, 2021·Physical Review. E·Sugam KumarVinod K Aswal
Oct 1, 2021·International Journal of Pharmaceutics·Hugo A L FilipeFilipe E Antunes
Dec 9, 2021·Journal of Occupational and Environmental Hygiene·Rachael L HardisonMegan W Howard

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Methods Mentioned

BETA
circular
X-ray
isothermal titration calorimetry
circular dichroism

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