PMID: 9438410Jan 23, 1998Paper

A completely biological tissue-engineered human blood vessel

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
N L'HeureuxFrançois A Auger

Abstract

Mechanically challenged tissue-engineered organs, such as blood vessels, traditionally relied on synthetic or modified biological materials for structural support. In this report, we present a novel approach to tissue-engineered blood vessel (TEBV) production that is based exclusively on the use of cultured human cells, i.e., without any synthetic or exogenous biomaterials. Human vascular smooth muscle cells (SMC) cultured with ascorbic acid produced a cohesive cellular sheet. This sheet was placed around a tubular support to produce the media of the vessel. A similar sheet of human fibroblasts was wrapped around the media to provide the adventitia. After maturation, the tubular support was removed and endothelial cells were seeded in the lumen. This TEBV featured a well-defined, three-layered organization and numerous extracellular matrix proteins, including elastin. In this environment, SMC reexpressed desmin, a differentiation marker known to be lost under standard culture conditions. The endothelium expressed von Willebrand factor, incorporated acetylated LDL, produced PGI2, and strongly inhibited platelet adhesion in vitro. The complete vessel had a burst strength over 2000 mmHg. This is the first completely biological TEB...Continue Reading

References

May 1, 1992·Annals of Vascular Surgery·K C KentA D Whittemore
Oct 1, 1991·The Journal of Investigative Dermatology·R FleischmajerL Y Sakai
Jan 1, 1990·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·G C LantzW E Blevins
Nov 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J ThybergB A Bottger
Jan 1, 1990·Annals of Vascular Surgery·H P Greisler
Dec 1, 1987·Annals of Vascular Surgery·J K DruryJ G Pollock
Feb 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M AbsherD Warshaw
Nov 1, 1985·The British Journal of Surgery·P M CharlesworthJ W Hallet
Aug 1, 1969·The Annals of Thoracic Surgery·C H Sparks
Jan 1, 1980·Biomaterials, Medical Devices, and Artificial Organs·R GuidoinP Blais
Nov 1, 1995·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·W TrubelP Polterauer
Dec 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·E LeeR T Lee
Sep 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F W Luscinskas, J Lawler
Jul 1, 1995·Journal of Biomedical Materials Research·M C HilesR J Morff
Apr 1, 1993·In Vitro Cellular & Developmental Biology. Animal·P W HewettM Woodcock
Nov 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C Y LuS C Sicher
Jan 1, 1994·Circulation·J G PickeringJ M Isner
May 1, 1993·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·G C LantzR J Morff
May 1, 1993·The Journal of Cell Biology·J R GambleM A Vadas
Jan 1, 1996·Nature Medicine·Y NoishikiA Matsumoto
Jun 1, 1995·In Vitro Cellular & Developmental Biology. Animal·F A AugerL Germain
Feb 1, 1996·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·C GillisA Haegerstrand

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Citations

Jan 23, 1999·Archives of Physiology and Biochemistry·G W BosJ Feijen
Jul 27, 1999·Journal of Biomedical Materials Research·A EberhartM W King
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S E Sakiyama-ElbertJ A Hubbell
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dorothy M SuppSteven T Boyce
Jan 19, 2002·Annals of the New York Academy of Sciences·I K Ko, H Iwata
Jun 26, 2002·Annals of the New York Academy of Sciences·Lucie GermainF A Auger
Jul 26, 2002·Artificial Organs·Kris DumontPascal R Verdonck
Feb 1, 2003·Plastic and Reconstructive Surgery·Oren M TepperGeoffrey C Gurtner
Jan 30, 2003·Vascular Medicine·Briain D MacNeillJoseph P Vacanti
Jun 27, 2003·The Journal of Craniofacial Surgery·Vladimir MironovRoger Markwald
Sep 19, 2003·Tissue Engineering·Dror SeliktarZorina S Galis
Sep 27, 2003·Tissue Engineering·Swee-Hin Teoh
Apr 29, 2004·Transplantation·Mark S DuxburyEdward E Whang
Jun 24, 2004·Vox Sanguinis·R M Nerem
Mar 12, 2005·Journal of Biomedical Materials Research. Part a·K L BilliarM T Frey
Aug 27, 2004·Médecine sciences : M/S·Anne Meddahi-PelléDidier Letourneur
Mar 2, 2005·Tissue Engineering·Guillaume GrenierLucie Germain
Mar 2, 2005·Tissue Engineering·Kee W NgDietmar W Hutmacher
Jul 7, 2005·Tissue Engineering·Gregory H BorschelDavid L Brown
Jul 7, 2005·Tissue Engineering·Yaakov NahmiasDavid J Odde
Aug 4, 2005·Expert Review of Cardiovascular Therapy·Chris D DalyJulie H Campbell
Feb 3, 2005·Circulation·Karina LaflammeLucie Germain
Aug 3, 2005·Annals of Biomedical Engineering·Chrysanthi Williams, Timothy M Wick
Nov 16, 2005·Cell Transplantation·Amy Solan, Laura Niklason
Dec 20, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·L ButtafocoJ Feijen
Aug 31, 2006·Biotechnology Journal·Thomas BolandXiaofeng Cui
Feb 24, 2006·Nature Medicine·Nicolas L'HeureuxTodd N McAllister
Sep 14, 2006·Tissue Engineering·Katia Bilodeau, Diego Mantovani
Aug 18, 2006·Head & Face Medicine·Bernhard FrerichAlexander Hemprich
Jun 26, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Cay M KieltyC Adrian Shuttleworth
Mar 9, 2007·World Journal of Surgery·Xinwen WangChangyi Chen
May 24, 2007·Tissue Engineering·Anthony P NapolitanoJeffrey R Morgan
Aug 21, 2007·Expert Opinion on Biological Therapy·Gabriel ZarbivMoshe Y Flugelman
May 15, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Nobuhiro NagaiMasanobu Munekata
Mar 13, 2008·Tissue Engineering. Part a·Josephine AllenGuillermo Ameer
May 6, 2008·Tissue Engineering. Part B, Reviews·Saejeong Kim, Horst von Recum

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