PMID: 9174192Jun 1, 1997Paper

A complex mutant of TEM-1 beta-lactamase with mutations encountered in both IRT-4 and extended-spectrum TEM-15, produced by an Escherichia coli clinical isolate

Antimicrobial Agents and Chemotherapy
D SirotJ Sirot

Abstract

Escherichia coli GR102 was isolated from feces of a leukemic patient. It expressed different levels of resistance to amoxicillin or ticarcillin plus clavulanate and to the various cephalosporins tested. The double-disk synergy test was weakly positive. Production of a beta-lactamase with a pI of 5.6 was transferred to E. coli HB101 by conjugation. The nucleotide sequence was determined by direct sequencing of the amplification products obtained by PCR performed with TEM gene primers. This enzyme differed from TEM-1 (blaT-1B gene) by four amino acid substitutions: Met-->Leu-69, Glu-->Lys-104, Gly-->Ser-238 and Asn-->Asp-276. The amino acid susbstitutions Leu-69 and Asp-276 are known to be responsible for inhibitor resistance of the IRT-4 mutant, as are Lys-104 and Ser-238 substitutions for hydrolytic activity of the extended-spectrum beta-lactamases TEM-15, TEM-4, and TEM-3. These combined mutations led to a mutant enzyme which conferred a level of resistance to coamoxiclav (MIC, 64 microg/ml) much lower than that conferred by IRT-4 (MIC, 2,048 microg/ml) but higher than that conferred by TEM-15 or TEM-1 (MIC, 16 microg/ml). In addition, the MIC of ceftazidime for E. coli transconjugant GR202 (1 microg/ml) was lower than that fo...Continue Reading

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Citations

Jun 20, 2000·Antimicrobial Agents and Chemotherapy·R BonnetJ Sirot
Feb 19, 2002·Antimicrobial Agents and Chemotherapy·Sergei Vakulenko, Dasantila Golemi
Nov 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert J HayesBassil I Dahiyat
Oct 25, 2003·Antimicrobial Agents and Chemotherapy·Corinne ArpinClaudine Quentin
Jan 28, 2005·The New England Journal of Medicine·George A Jacoby, Luisa Silvia Munoz-Price
Aug 22, 2007·Antimicrobial Agents and Chemotherapy·Frédéric RobinRichard Bonnet
Nov 11, 2008·Applied and Environmental Microbiology·Karen L LachmayrTimothy E Ford
Dec 22, 2010·Antimicrobial Agents and Chemotherapy·Frédéric RobinRichard Bonnet
Oct 18, 2002·Antimicrobial Agents and Chemotherapy·William A SchroederSusan E Jensen
Nov 25, 2004·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Jun 28, 2006·Antimicrobial Agents and Chemotherapy·Frédéric RobinRichard Bonnet
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·George A Jacoby
Jan 26, 2013·Annals of the New York Academy of Sciences·David M Shlaes
Dec 10, 2009·Antimicrobial Agents and Chemotherapy·Karen Bush, George A Jacoby
Aug 20, 2014·Antimicrobial Agents and Chemotherapy·Francisco José Pérez-LlarenaGermán Bou
Jun 1, 1999·The Journal of Antimicrobial Chemotherapy·E B ChaïbiR Labia
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·Chedly ChouchaniKamel Ben-Mahrez
Jun 27, 2015·Molecular Biology and Evolution·Portia M MiraMiriam Barlow
Feb 15, 2001·Current Infectious Disease Reports·Patricia A. Bradford
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·V Leflon-GuiboutM Nicolas-Chanoine
Jan 13, 2010·Clinical Microbiology Reviews·Sarah M Drawz, Robert A Bonomo
Oct 15, 2005·Clinical Microbiology Reviews·David L Paterson, Robert A Bonomo
Apr 28, 2010·Antimicrobial Agents and Chemotherapy·Krisztina M Papp-WallaceRobert A Bonomo
Oct 30, 1999·Antimicrobial Agents and Chemotherapy·R BonnetJ Sirot
Dec 19, 2020·Frontiers in Molecular Biosciences·Thinh-Phat CaoSung Haeng Lee
Jan 30, 1999·Antimicrobial Agents and Chemotherapy·S Goussard, P Courvalin
Jan 21, 2021·Antimicrobial Agents and Chemotherapy·Portia M MiraMiriam Barlow
Feb 4, 1998·Antimicrobial Agents and Chemotherapy·I Massova, S Mobashery

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