A comprehensive analysis of pattern recognition receptors in normal and inflamed human epidermis: upregulation of dectin-1 in psoriasis

The Journal of Investigative Dermatology
Heleen D de KoningPatrick L J M Zeeuwen

Abstract

Human epidermis plays an important role in host defense by acting as a physical barrier and signaling interface between the environment and the immune system. Pattern recognition receptors (PRRs) are crucial to maintain homeostasis and provide protection during infection, but are also causally involved in monogenic auto-inflammatory diseases. This study aimed to investigate the epidermal expression of PRRs and several associated host defense molecules in healthy human skin, psoriasis, and atopic dermatitis (AD). Using microarray analysis and real-time quantitative PCR, we found that many of these genes are transcribed in normal human epidermis. Only a few genes were differentially induced in psoriasis (CLEC7A (dectin-1), Toll-like receptor (TLR) 4, and mannose receptor C type 1 (MRC1)) or AD (MRC1, IL1RN, and IL1β) compared with normal epidermis. A remarkably high expression of dectin-1 mRNA was observed in psoriatic epidermis and this was corroborated by immunohistochemistry. In cultured primary human keratinocytes, dectin-1 expression was induced by IFN-γ, IFN-α, and Th17 cytokines. Keratinocytes were unresponsive, however, to dectin-1 ligands such as β-glucan or heat-killed Candida albicans, nor did we observe synergy with T...Continue Reading

References

Aug 1, 1996·Journal of Cellular Physiology·F Van RuissenJ Schalkwijk
Aug 21, 2001·The Journal of Investigative Dermatology·G SzolnokyL Kemény
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 23, 2002·The Journal of Investigative Dermatology·Peter I SongJohn C Ansel
Oct 11, 2002·The New England Journal of Medicine·Peck Y OngDonald Y M Leung
Nov 22, 2002·Journal of Dermatological Science·Kazuhiro KawaiMasaaki Ito
Apr 30, 2003·The Journal of Experimental Medicine·Benjamin N GantnerDavid M Underhill
May 17, 2003·International Immunology·Andor PivarcsiLajos Kemény
Jan 20, 2004·The Journal of Biological Chemistry·Katalin KarikóDrew Weissman
Dec 16, 2005·The Journal of Investigative Dermatology·Gys J de JonghJoost Schalkwijk
Oct 28, 2006·The Journal of Investigative Dermatology·Maria C LebreEsther C de Jong
Dec 14, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Alain KummerJürg Tschopp
Jan 18, 2007·The Journal of Investigative Dermatology·Lloyd S Miller, Robert L Modlin
Feb 6, 2007·Seminars in Immunology·Sean R Christensen, Mark J Shlomchik
Apr 24, 2007·Nature Immunology·Salomé LeibundGut-LandmannCaetano Reis e Sousa
Sep 18, 2007·Current Opinion in Allergy and Clinical Immunology·Michael D Howell
Jan 18, 2008·European Journal of Immunology·Kevin M DennehyGordon D Brown
Jul 31, 2008·Nature Clinical Practice. Rheumatology·Bénédicte NevenPierre Quartier dit Maire
Oct 8, 2008·The Journal of Experimental Medicine·Karen A CavassaniSteven L Kunkel
Jan 6, 2009·Immunological Reviews·Luigi FranchiGabriel Nuñez
Jan 6, 2009·Immunological Reviews·Noah W Palm, Ruslan Medzhitov
Jan 6, 2009·The Journal of Investigative Dermatology·Jürgen Harder, Gabriel Núñez
Feb 19, 2009·Current Opinion in Immunology·Delyth M ReidGordon D Brown
Feb 21, 2009·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·M Meurer
Feb 28, 2009·International Immunology·Taro Kawai, Shizuo Akira
Mar 27, 2009·The Journal of Investigative Dermatology·Antonella Di CesareFrank O Nestle
Apr 16, 2009·Clinical Microbiology Reviews·Trine H Mogensen
Apr 22, 2009·Cell Host & Microbe·Frank L van de VeerdonkMihai G Netea
Jun 27, 2009·Nature Reviews. Immunology·Gregory M Barton, Jonathan C Kagan
Jul 31, 2009·The New England Journal of Medicine·Frank O NestleJonathan Barker
Oct 30, 2009·The New England Journal of Medicine·Erik-Oliver GlockerBodo Grimbacher
Oct 30, 2009·The New England Journal of Medicine·Bart FerwerdaMihai G Netea
Jan 29, 2010·The Journal of Investigative Dermatology·Jürgen HarderRegine Gläser

❮ Previous
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Citations

May 22, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Wynette M DietzMark J Osborn
Jul 22, 2011·Clinical & Developmental Immunology·David L Moyes, Julian R Naglik
Nov 17, 2012·Genome Biology·Patrick L J M ZeeuwenHarro M Timmerman
Dec 23, 2015·Experimental Eye Research·Tohru Sakimoto, Akiko Ishimori
Feb 5, 2013·The Journal of Allergy and Clinical Immunology·I-Hsin KuoLisa A Beck
May 25, 2012·The Journal of Investigative Dermatology·Judith G M BergboerJoost Schalkwijk
Jan 6, 2012·The Journal of Investigative Dermatology·Danuta Gutowska-Owsiak, Graham S Ogg
Jul 29, 2011·The Journal of Investigative Dermatology·Carren Sy HauShinichi Sato
Jan 25, 2012·The Journal of Pathology·H de la FuenteFrancisco Sanchez-Madrid
Apr 21, 2012·Microbes and Infection·Heleen D de KoningJoost Schalkwijk
Aug 2, 2011·Microbes and Infection·Julian R NaglikBernhard Hube
Jul 23, 2015·Arthritis Research & Therapy·Heleen D de KoningAnna Simon
Oct 15, 2013·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·L FellerJ Lemmer
Oct 8, 2016·Immunology and Cell Biology·Sachin SharmaSubhrajit Biswas
Mar 9, 2012·Journal of Innate Immunity·Heleen D de KoningJoost Schalkwijk
Feb 4, 2019·Clinical and Experimental Immunology·C HagertR Holmdahl
Mar 12, 2011·Nature Reviews. Immunology·Luigina Romani
Apr 26, 2019·PloS One·A Lenore AckermanUNKNOWN NIH Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP)
Nov 21, 2012·Experimental Dermatology·Patrick A M JansenPatrick L J M Zeeuwen
Nov 23, 2012·Experimental Dermatology·Maria CarlströmCharlotta Enerbäck
Sep 22, 2020·Applied in Vitro Toxicology·James GowChangjiang Guo
Mar 5, 2013·Journal of the European Academy of Dermatology and Venereology : JEADV·S Garcia-Rodriguez M Zubiaur
Nov 1, 2020·Journal of Cellular and Molecular Medicine·Valerio CaputoRaffaella Cascella
Dec 10, 2020·International Journal of Molecular Sciences·Lucian BeerMichael Mildner
Mar 29, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Aneesh V KarkhanisEe Chee Ren
Jun 13, 2021·Journal of Dermatological Science·Thomas LugerMatthias Schmuth

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