Nov 5, 2018

A comprehensive analysis of single-cell transcriptome network underlying gastric premalignant lesions and early gastric cancer

BioRxiv : the Preprint Server for Biology
Zhang PengLi Shao

Abstract

Intestinal-type gastric cancer is preceded by premalignant lesions including chronic atrophic gastritis (CAG) and intestinal metaplasia (IM), which are characterized as changes in cell types. In this study, for the first time, we systematically constructed a single-cell atlas for a total of 31,164 high-quality cells from gastric mucosa biopsies of patients spanning a cascade of gastric premalignant lesions and early gastric cancer (EGC) using single-cell RNA sequencing (scRNA-seq). Based on the atlas, we construct a network underlying the changes of cellular and molecular characteristics of gastric epithelial cells across different lesions. We found the conversion of gland mucous cells (GMCs) toward a more intestinal-like stem cell phenotype during metaplasia, and identified OR51E1 as a novel marker for early-malignant enteroendocrine cells. We also found that HES6 might mark a goblet cell subset that precede morphologically identifiable goblet cells in IM mucosa, potentially aiding the identification of metaplasia at the early stage. Finally, we identified a panel of EGC-related specific signature, with clinical implications for the precise diagnosis of EGC. Our study offers unparalleled insights into the human gastric cellulo...Continue Reading

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Mentioned in this Paper

Biological Markers
Study
Chorionic alpha(2)-microglobulin
Malignant Neoplasm of Stomach
Stomach Carcinoma
Sequence Determinations, RNA
Squamous Transitional Epithelial Cell Count
Precancerous Conditions
Sequence Determinations
OR51E1

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Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.