A comprehensive analysis of the microbial communities of healthy and diseased marine macroalgae and the detection of known and potential bacterial pathogens

Frontiers in Microbiology
Enrique Zozaya-ValdesTorsten Thomas

Abstract

Microorganisms are increasingly being recognized as the causative agents in the diseases of marine higher organisms, such as corals, sponges, and macroalgae. Delisea pulchra is a common, temperate red macroalga, which suffers from a bleaching disease. Two bacterial strains, Nautella italica R11 and Phaeobacter gallaeciensis LSS9, have been shown in vitro to cause bleaching symptoms, but previous work has failed to detect them during a natural bleaching event. To provide a link between in vitro observations and natural occurrences of the disease, we employ here deep-sequencing of the 16S rRNA gene to comprehensively analyze the community composition of healthy and diseased D. pulchra samples from two separate locations. We observed operational taxonomic units (OTUs) with 100% identity and coverage to the 16S RNA gene sequence of both in vitro pathogens, but only the OTU with similarity to strain LSS9 showed a statistically significant higher abundance in diseased samples. Our analysis also reveals the existence of other bacterial groups within the families Rhodobacteraceae and Flavobacteriaceae that strongly contribute to difference between diseased and healthy samples and thus these groups potentially contain novel macroalgal p...Continue Reading

References

Apr 26, 2003·Environmental Microbiology·Olga PantosJohn C Bythell
Apr 30, 2003·Canadian Journal of Microbiology·Gou FurusawaTaizo Sakata
May 12, 2004·The Journal of Eukaryotic Microbiology·Xavier Mayali, Farooq Azam
Oct 6, 2005·Applied and Environmental Microbiology·Alison BuchanMary Ann Moran
May 25, 2006·Annual Review of Microbiology·Irene Wagner-Döbler, Hanno Biebl
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Mar 27, 2007·Nature Reviews. Microbiology·Eugene RosenbergIlana Zilber-Rosenberg
Feb 7, 2008·Archives of Microbiology·Thorsten BrinkhoffMeinhard Simon
Nov 14, 2008·The ISME Journal·Eugene RosenbergLoya Yossi
Apr 29, 2009·Environmental Microbiology·Rebecca Vega ThurberForest Rohwer
Oct 14, 2009·Trends in Microbiology·David G BourneC Drew Harvell
Dec 17, 2009·Trends in Ecology & Evolution·Tracy D AinsworthRuth D Gates
Jan 22, 2010·The ISME Journal·Anna EngelbrektsonPhilip Hugenholtz
Oct 16, 2010·Environmental Microbiology·Rebecca J CaseStaffan Kjelleberg
Dec 2, 2010·FEMS Microbiology Ecology·Hilde AngermeierUte Hentschel
Feb 10, 2011·Standards in Genomic Sciences·Jack A GilbertRick Stevens
May 12, 2011·Integrative and Comparative Biology·Morgan E MouchkaC Drew Harvell
May 31, 2011·International Journal of Systematic and Evolutionary Microbiology·Bokun LinZhong Hu
Jun 23, 2011·BMC Genomics·Daniela KalhoeferThorsten Brinkhoff
Jul 19, 2011·Archives of Microbiology·Wen-Ming ChenShih-Yi Sheu
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Catherine BurkeTorsten Thomas
Apr 27, 2012·Journal of Bacteriology·Bokun LinHonglin Chen
Jul 11, 2012·FEMS Microbiology Ecology·Joke HollantsAnne Willems
Nov 20, 2012·FEMS Microbiology Reviews·Suhelen EganTorsten Thomas
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Dec 4, 2012·Nature Methods·Nicholas A BokulichJ Gregory Caporaso
Dec 1, 2011·Environmental Microbiology Reports·Raechel LittmanDavid G Bourne
Oct 12, 2013·Environmental Microbiology·Suhelen EganTorsten Thomas
Nov 30, 2013·Nucleic Acids Research·James R ColeJames M Tiedje

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Citations

Jan 19, 2016·Frontiers in Microbiology·Tim LachnitPeter Steinberg
Jan 19, 2016·Frontiers in Microbiology·Ravindra Pal Singh, C R K Reddy
Jul 3, 2015·Frontiers in Microbiology·Suhelen Egan, Torsten Thomas
Jul 26, 2016·Frontiers in Microbiology·Suhelen Egan, Melissa Gardiner
Jun 24, 2016·Environmental Microbiology·Vipra KumarSuhelen Egan
Apr 19, 2017·Environmental Microbiology·Enrique Zozaya-ValdésTorsten Thomas
Jan 10, 2018·Frontiers in Microbiology·July Z FlorezAlejandro H Buschmann
Jan 26, 2018·NPJ Biofilms and Microbiomes·Ezequiel M MarzinelliMariana Mayer-Pinto
Apr 14, 2020·MicrobiologyOpen·Charles G Lewis, Melanie J Beazley
Feb 23, 2020·Genes·Ben J NovakThomas H Maloney
Jul 28, 2017·Molecular Ecology·Vanessa Rossetto MarcelinoHeroen Verbruggen
Aug 17, 2019·Frontiers in Microbiology·Anne TournerocheSoizic Prado
May 30, 2019·Frontiers in Microbiology·Valentina Hurtado-McCormickJustin Robert Seymour
Apr 6, 2021·Environmental Microbiology·Katherine M DavisLaura Wegener Parfrey
Aug 4, 2021·The ISME Journal·Jiasui LiSuhelen Egan
Sep 28, 2021·Journal of Phycology·July Z FlorezAlejandro H Buschmann

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Datasets Mentioned

BETA
SRX824554
SRX824555

Methods Mentioned

BETA
electrophoresis

Software Mentioned

Mothur
Copyrighter
QIIME
blastn
Primer
InvSimpson
mvabund
RDP
Greengenes
Silva

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