A comprehensive analysis of translational missense errors in the yeast Saccharomyces cerevisiae.

RNA
Emily B KramerPhilip J Farabaugh

Abstract

The process of protein synthesis must be sufficiently rapid and sufficiently accurate to support continued cellular growth. Failure in speed or accuracy can have dire consequences, including disease in humans. Most estimates of the accuracy come from studies of bacterial systems, principally Escherichia coli, and have involved incomplete analysis of possible errors. We recently used a highly quantitative system to measure the frequency of all types of misreading errors by a single tRNA in E. coli. That study found a wide variation in error frequencies among codons; a major factor causing that variation is competition between the correct (cognate) and incorrect (near-cognate) aminoacyl-tRNAs for the mutant codon. Here we extend that analysis to measure the frequency of missense errors by two tRNAs in a eukaryote, the yeast Saccharomyces cerevisiae. The data show that in yeast errors vary by codon from a low of 4 x 10(-5) to a high of 6.9 x 10(-4) per codon and that error frequency is in general about threefold lower than in E. coli, which may suggest that yeast has additional mechanisms that reduce missense errors. Error rate again is strongly influenced by tRNA competition. Surprisingly, missense errors involving wobble positio...Continue Reading

References

Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H J GrosjeanD M Crothers
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R C Thompson, P J Stone
Jan 1, 1975·Biochimie·J Ninio
Sep 1, 1989·Microbiological Reviews·J Parker
Mar 1, 1988·Trends in Biochemical Sciences·R C Thompson
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J J Hopfield
Oct 1, 1973·Antimicrobial Agents and Chemotherapy·R Benveniste, J Davies
Mar 14, 1969·Journal of Molecular Biology·E SchweizerH O Halvorson
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L E AlksneJ R Warner
May 2, 1997·Journal of Molecular Biology·R PercudaniS Ottonello
Apr 25, 1997·Biochimica Et Biophysica Acta·L YeF R Leach
Sep 12, 1998·Journal of Molecular Biology·I StansfieldM F Tuite
Jun 5, 1999·The EMBO Journal·M I RechtJ D Puglisi
Aug 31, 2000·Annual Review of Biochemistry·M Ibba, D Soll
May 27, 2003·Trends in Biochemical Sciences·James M OgleV Ramakrishnan
Oct 1, 2004·Molecular and Cellular Biology·Magdalena Rakwalska, Sabine Rospert
Feb 1, 2005·FEBS Letters·Marina V RodninaHans-Joachim Wieden
Jun 15, 2005·Annual Review of Biochemistry·James M Ogle, V Ramakrishnan
Feb 4, 2006·Molecular Cell·Kirill B GromadskiMarina V Rodnina
Jul 31, 2007·Nature Structural & Molecular Biology·Maria A BorovinskayaJamie H Doudna Cate
Dec 19, 2008·Nature·Hani S Zaher, Rachel Green

❮ Previous
Next ❯

Citations

Jun 6, 2014·PLoS Genetics·Fabienne F V ChevanceKelly T Hughes
Apr 30, 2014·Annual Review of Genomics and Human Genetics·Kim M KeelingDavid M Bedwell
Jul 21, 2014·Cellular and Molecular Life Sciences : CMLS·Daniel Tarrant, Tobias von der Haar
Nov 14, 2014·Molecular Biology of the Cell·Jadyn R DamonHidde L Ploegh
Aug 16, 2011·The Journal of Clinical Investigation·Fan-Yan WeiKazuhito Tomizawa
Jun 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tanja MattErik C Böttger
Mar 16, 2013·Molecular Biology and Evolution·Edward W J WallaceD Allan Drummond
May 30, 2013·Molecular and Cellular Biology·Tek N LamichhaneRichard J Maraia
Jul 23, 2014·PLoS Biology·Jian-Rong YangJianzhi Zhang
May 22, 2013·Journal of Molecular Evolution·Tugce BilginAndreas Wagner
May 28, 2014·Journal of Molecular Biology·Benjamin P Roscoe, Daniel N A Bolon
Apr 30, 2015·Biochimie·Adélaïde Saint-Léger, Lluís Ribas de Pouplana
Jan 13, 2015·Nucleic Acids Research·Yongqiang FanJiqiang Ling
Apr 22, 2009·PloS One·Raquel M SilvaManuel A S Santos
Jun 2, 2016·DNA Repair·Brian MagnusonMats Ljungman
Mar 1, 2012·PloS One·Smita AmarnathVidyanand Nanjundiah
Nov 22, 2007·PloS One·Di WuAlan Michael Tartakoff
Apr 6, 2012·PLoS Genetics·Wenfeng QianJianzhi Zhang
Jul 30, 2016·Scientific Reports·Yongqiang FanJiqiang Ling
Aug 31, 2013·Annual Review of Genetics·Tao Pan
Jun 30, 2016·Human Molecular Genetics·Anton A Komar
Nov 1, 2016·Nature Structural & Molecular Biology·Kanghyun LeeElizabeth A Craig
Jan 12, 2017·Critical Reviews in Biochemistry and Molecular Biology·Michael H Schwartz, Tao Pan
Feb 6, 2017·Biochimica Et Biophysica Acta. General Subjects·Kyle S HoffmanChristopher J Brandl
Mar 16, 2017·Trends in Genetics : TIG·Christina E Brule, Elizabeth J Grayhack
Apr 5, 2017·Bulletin of Mathematical Biology·Annwesha Dutta, Debashish Chowdhury
Jan 20, 2017·Open Biology·Tobias von der HaarCampbell W Gourlay
Feb 1, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marina V RodninaHolger Stark
Mar 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Sandra BlanchetOlivier Namy
Sep 22, 2017·RNA Biology·Jeremy T LantPatrick O'Donoghue
Oct 12, 2017·Nature Reviews. Molecular Cell Biology·Gavin Hanson, Jeff Coller
Apr 4, 2018·Cold Spring Harbor Perspectives in Biology·Thomas E DeverRachel Green
Aug 22, 2018·Expert Review of Respiratory Medicine·Oliver J McElvaneyNoel G McElvaney
Dec 18, 2018·Yeast·Kartikeya JoshiPhilip J Farabaugh
Dec 7, 2018·The Journal of Biological Chemistry·Kevin C Stein, Judith Frydman
Jun 8, 2012·Critical Reviews in Biochemistry and Molecular Biology·Kim M KeelingDavid M Bedwell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.