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A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples

BMC Genomics

Jun 7, 2017

Sven SchuiererGuglielmo Roma

Abstract

RNA-sequencing (RNA-seq) has emerged as one of the most sensitive tool for gene expression analysis. Among the library preparation methods available, the standard poly(A) + enrichment provides a comprehensive, detailed, and accurate view of polyadenylated RNAs. However, on samples of su...read more

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Exons
Sequence Determinations, RNA
RNA, Polyadenylated
Ribosomal RNA
Whole Exome Sequencing
Gene Expression
Evaluation
Poly A
Sequencing
15
142
Paper Details
References

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A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples

BMC Genomics

Jun 7, 2017

Sven SchuiererGuglielmo Roma

PMID: 28583074

DOI: 10.1186/s12864-017-3827-y

Abstract

RNA-sequencing (RNA-seq) has emerged as one of the most sensitive tool for gene expression analysis. Among the library preparation methods available, the standard poly(A) + enrichment provides a comprehensive, detailed, and accurate view of polyadenylated RNAs. However, on samples of su...read more

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Exons
Sequence Determinations, RNA
RNA, Polyadenylated
Ribosomal RNA
Whole Exome Sequencing
Gene Expression
Evaluation
Poly A
Sequencing
15
142

Similar Papers Found In These Feeds

Comparative Sequencing

Inter/Intra-Species: Comparative Sequencing describes the sequencing techniques that compare between and within species genomes. Discover the latest research on comparative sequencing of inter/intra-species here.

Perturb-seq

Perturb-seq has combined CRISPR gene inactivation and single-cell rna-sequencing (scRNA-seq) and is the newest addition to the geneticist's arsenal, providing scientists with study methods for functional genomics. Discover the latest research here.

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