A comprehensive characterization of novel CYP-BM3 homolog (CYP-BA) from Bacillus aryabhattai.

Enzyme and Microbial Technology
Sourya BhattacharyaSaugata Hazra

Abstract

Functionalizing C-H bond poses one of the most significant challenges for chemists providing them with very few substrate-specific synthetic routes. Despite being incredibly plastic in their enzymatic ability, they are confined with deficient enzymatic action and limited explicitness of the substrates. In this study, we have endeavored to characterize novel cytochrome P450 from Bacillus aryabhattai (CYP-BA), a homolog of CYP P450-BM3, by taking interdisciplinary approaches. We conducted structure and sequence comparison to understand the conservation pattern for active site residues, conserved fold, evolutionary relationships among others. Molecular dynamics simulations were performed to understand the dynamic nature and interaction with the substrates. CYP-BA was successfully cloned, purified, and characterized. The enzyme's stability toward various physicochemical parameters was evaluated by UV-vis spectroscopy and Circular Dichroism (CD) spectroscopy. Various saturated fatty acids being the natural cytochrome P450 substrates were evaluated as catalytic efficiency of substrate oxidation by CYP-BA. The binding affinity of these natural substrates was monitored against CYP-BA by isothermal titration calorimetry (ITC). The catal...Continue Reading

References

Sep 15, 1976·Journal of Molecular Biology·K KuwajimaS Sugai
Jun 1, 1992·Computer Applications in the Biosciences : CABIOS·D T JonesJ M Thornton
Dec 1, 1993·Proteins·A AmadeiH J Berendsen
Jan 12, 2002·Current Opinion in Structural Biology·S Leavitt, E Freire
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Sep 9, 2004·Chemical Reviews·Bernard MeunierSason Shaik
Jun 12, 2009·Journal of Virology·David C LambSteven L Kelly
Sep 24, 2009·Chemistry : a European Journal·Andrew M SawayamaFrances H Arnold
May 3, 2011·European Biophysics Journal : EBJ·Nathan SchmidWilfred F van Gunsteren
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Oct 20, 2011·Chemical Society Reviews·Christopher J C WhitehouseLuet-Lok Wong
Jan 31, 2014·Journal of Computer-aided Molecular Design·Katarzyna B KoziaraAlan E Mark
May 23, 2014·Journal of Chemical Information and Modeling·Rashmi KumariAndrew Lynn
Dec 13, 2011·Journal of Chemical Theory and Computation·Alpeshkumar K MaldeAlan E Mark
May 10, 2011·Journal of Chemical Theory and Computation·Wei HuangWilfred F van Gunsteren
Feb 1, 2012·Molecular Informatics·Nadine Homeyer, Holger Gohlke
Oct 7, 2016·Nature Communications·Kathryn M HartGregory R Bowman
May 4, 2018·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Nov 27, 2018·International Journal of Biological Macromolecules·Sayak BhattacharyaJoydeep Mukherjee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.