A Comprehensive Genome Survey Provides Novel Insights into Bile Salt Hydrolase (BSH) in Lactobacillaceae

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Lifeng LiangGengyun Zhang

Abstract

Bile salt hydrolase (BSH) is a well-known enzyme that has been commonly characterized in probiotic bacteria, as it has cholesterol-lowering effects. However, its molecular investigations are scarce. Here, we build a local database of BSH sequences from Lactobacillaceae (BSH⁻SDL), and phylogenetic analysis and homology searches were employed to elucidate their comparability and distinctiveness among species. Evolutionary study demonstrates that BSH sequences in BSH⁻SDL are divided into five groups, named BSH A, B, C, D and E here, which can be the genetic basis for BSH classification and nomenclature. Sequence analysis suggests the differences between BSH-active and BSH-inactive proteins clearly, especially on site 82. In addition, a total of 551 BSHs from 107 species are identified from 451 genomes of 158 Lactobacillaceae species. Interestingly, those bacteria carrying various copies of BSH A or B can be predicted to be potential cholesterol-lowering probiotics, based on the results of phylogenetic analysis and the subtypes that those previously reported BSH-active probiotics possess. In summary, this study elaborates the molecular basis of BSH in Lactobacillaceae systematically, and provides a novel methodology as well as a co...Continue Reading

References

Jan 1, 1972·The Journal of Clinical Investigation·K E AndersonN B Javitt
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Aug 9, 2005·Applied and Environmental Microbiology·Olivia McAuliffeTodd R Klaenhammer
Jan 27, 2007·Journal of Industrial Microbiology & Biotechnology·Sahar F DerazBo Mattiasson
Jun 10, 2008·Applied and Environmental Microbiology·Jolanda M LambertMichiel Kleerebezem
Mar 10, 2009·International Journal of Epidemiology·Crystal Man Ying LeeUNKNOWN Asia Pacific Cohort Studies Collaboration
Jul 18, 2009·World Journal of Gastroenterology : WJG·Harris BernsteinKaterina Dvorak
Jul 20, 2010·International Journal of Molecular Sciences·Lay-Gaik Ooi, Min-Tze Liong
Jan 27, 2011·Archives of Toxicology·Carol BernsteinHarris Bernstein
Feb 22, 2011·Bioinformatics·Diego DarribaDavid Posada
Sep 21, 2011·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Z GuoW Chen
May 23, 2012·Experimental Diabetes Research·Manoj KumarHariom Yadav
Aug 8, 2014·Applied Biochemistry and Biotechnology·Sathyanarayanan JayashreeParamasamy Gunasekaran
Jun 9, 2016·International Journal of Cancer. Journal International Du Cancer·Guoxiang XieWei Jia
Oct 23, 2016·Biochimica Et Biophysica Acta. General Subjects·Deepak ChandSureshkumar Ramasamy
Oct 12, 2017·Nature Reviews. Gastroenterology & Hepatology·Wei JiaWeiping Jia
Dec 3, 2017·Trends in Endocrinology and Metabolism : TEM·Antonio MolinaroHanns-Ulrich Marschall

❮ Previous
Next ❯

Software Mentioned

BLASTP
Prottest
Python script
iToL
PhyML
house
MUSCLE
TBtools
TeXshade

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.