A comprehensive HPLC analytical system for the identification and quantification of hexoses that employs 2-aminobenzamide coupling

Journal of Biochemistry
Kayo HasehiraJun Hirabayashi

Abstract

Rare sugars are defined as monosaccharides with extremely low natural abundances. Their natural distributions and biological functions remain to be clarified. To establish a robust analytical system that can separate, identify and quantify rare sugars, 12 d-hexoses-including five rare aldohexoses and three rare ketohexoses-were labelled with 2-aminobenzamide (2-AB), and the fluorescently tagged monosaccharides were separated by high-performance liquid chromatography (HPLC). Because the ketohexoses were much less reactive than were the aldohexoses, the reaction conditions were optimized to achieve the maximum yields (>75%) for both aldohexoses and ketohexoses. The calibration curve determined for the rare ketohexose, d-psicose (Psi), was linear between 1 pmol and 1 micromol and had a correlation coefficient of 0.9999. Using the developed method, the psicose content in a leaf of Itea virginica, in which the presence of psicose has previously been reported, was found to be 2.7 mg psicose/g leaf. The result proved feasibility of the method even for natural products. Because the system is a comprehensive tool, it should help answer questions concerning the biosyntheses and functions of rare hexose sugars.

References

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Jan 29, 2008·Biosensors & Bioelectronics·Nobumitsu MiyanishiJun Hirabayashi

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Citations

Jul 11, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Mami AkabaneShuji Kodama

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