A comprehensive investigation of starch degradation process and identification of a transcriptional activator MabHLH6 during banana fruit ripening

Plant Biotechnology Journal
Yun-Yi XiaoWang-Jin Lu

Abstract

Although starch degradation has been well studied in model systems such as Arabidopsis leaves and cereal seeds, this process in starchy fruits during ripening, especially in bananas, is largely unknown. In this study, 38 genes encoding starch degradation-related proteins were identified and characterized from banana fruit. Expression analysis revealed that 27 candidate genes were significantly induced during banana fruit ripening, with concomitant conversion of starch-to-sugars. Furthermore, iTRAQ-based proteomics experiments identified 18 starch degradation-associated enzymes bound to the surface of starch granules, of which 10 were markedly up-regulated during ripening. More importantly, a novel bHLH transcription factor, MabHLH6, was identified based on a yeast one-hybrid screening using MaGWD1 promoter as a bait. Transcript and protein levels of MabHLH6 were also increased during fruit ripening. Electrophoretic mobility shift assays, chromatin immunoprecipitation and transient expression experiments confirmed that MabHLH6 activates the promoters of 11 starch degradation-related genes, including MaGWD1, MaLSF2, MaBAM1, MaBAM2, MaBAM8, MaBAM10, MaAMY3, MaAMY3C, MaISA2, MaISA3 and MapGlcT2-2 by recognizing their E-box (CANNTG)...Continue Reading

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Citations

Apr 29, 2018·Physiologia Plantarum·Yu-Fan GuoJian-Fei Kuang
Apr 20, 2019·Frontiers in Plant Science·Beatriz Rosana Cordenunsi-LysenkoFernanda Helena Gonçalves Peroni-Okyta
Mar 7, 2020·Frontiers in Plant Science·Stefano BrizzolaraPietro Tonutti
Feb 20, 2019·Frontiers in Plant Science·Anna Maria SalzanoAndrea Scaloni
May 12, 2020·The Plant Journal : for Cell and Molecular Biology·Tao ZhangKaidong Liu
Sep 14, 2020·Plant Biotechnology Journal·Jian-Fei KuangWang-Jin Lu
Oct 3, 2020·Journal of the Science of Food and Agriculture·Rafaela SantosCatarina Prista
Dec 16, 2020·International Journal of Molecular Sciences·Wei WeiWei Shan
Oct 14, 2020·The Plant Journal : for Cell and Molecular Biology·Haohao CaoZhengguo Li
Feb 6, 2021·Journal of Experimental Botany·Kaijie ZhuXiuxin Deng
Mar 30, 2021·International Journal of Food Science & Technology·Jhon Larry MorenoDominique Dufour
Mar 4, 2021·Journal of Integrative Plant Biology·Guoxiang JiangXuewu Duan
Jul 21, 2021·Journal of Experimental Botany·Juhua LiuZhiqiang Jin
Sep 1, 2021·Journal of Agricultural and Food Chemistry·Ying QiJunfeng Guan
Oct 19, 2021·Plant Physiology·Laure C DavidSamuel C Zeeman

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Methods Mentioned

BETA
scanning electron microscopy
one‐hybrid
PCR
affinity purification
Assay
protein assay
electrophoretic
immunoprecipitation

Software Mentioned

MeV

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