A comprehensive investigation of the mRNA and protein level of ACE2, the putative receptor of SARS-CoV-2, in human tissues and blood cells

International Journal of Medical Sciences
Yiliang WangYifei Wang

Abstract

The outbreak of pneumonia caused by SARS-CoV-2 posed a great threat to global human health, which urgently requires us to understand comprehensively the mechanism of SARS-CoV-2 infection. Angiotensin-converting enzyme 2 (ACE2) was identified as a functional receptor for SARS-CoV-2, distribution of which may indicate the risk of different human organs vulnerable to SARS-CoV-2 infection. Previous studies investigating the distribution of ACE2 mRNA in human tissues only involved a limited size of the samples and a lack of determination for ACE2 protein. Given the heterogeneity among humans, the datasets covering more tissues with a larger size of samples should be analyzed. Indeed, ACE2 is a membrane and secreted protein, while the expression of ACE2 in blood and common blood cells remains unknown. Herein, the proteomic data in HIPED and the antibody-based immunochemistry result in HPA were collected to analyze the distribution of ACE2 protein in human tissues. The bulk RNA-seq profiles from three separate public datasets including HPA tissue Atlas, GTEx, and FANTOM5 CAGE were also obtained to determine the expression of ACE2 in human tissues. Moreover, the abundance of ACE2 in human blood and blood cells was determined by analyzi...Continue Reading

Citations

Aug 28, 2020·Frontiers in Pediatrics·Yun WangRuiman Li
Aug 23, 2020·The Pediatric Infectious Disease Journal·Angelo Di Vincenzo, Marco Rossato
Oct 13, 2020·Fetal and Pediatric Pathology·Mai HeLouis P Dehner
Oct 27, 2020·Frontiers in Microbiology·Jie ZhaoBao-Ping Tian
Nov 11, 2020·Lung·Filippo ScialoAndrea Bianco
Jan 7, 2021·Stem Cell Reviews and Reports·Irma Virant-Klun, Franc Strle
Jan 19, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Murat Oz, Dietrich Ernst Lorke
Jan 1, 2021·Cell & Bioscience·Miriam Marlene Medina-EnríquezTalia Wegman-Ostrosky
Nov 26, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Girish PathangeyAmr E Abbas
Mar 5, 2021·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Brendin FlinnNalini Santanam
May 24, 2021·The American Journal of the Medical Sciences·Sohaib AshrafMateen Izhar
Jun 6, 2021·Molecular & Cellular Proteomics : MCP·Jeremy L Praissman, Lance Wells
Apr 14, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Branka Miličić StanićKathryn Sandberg

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
flow cytometry
Fluorescence-Activated Cell Sorting

Related Concepts

Related Feeds

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Related Papers

BioRxiv : the Preprint Server for Biology
Jessie Low-GanVaughn Smider
BioRxiv : the Preprint Server for Biology
J. L. GanVaughn V. Smider
Frontiers in Molecular Biosciences
Federica SaponaroRiccardo Zucchi
© 2021 Meta ULC. All rights reserved