A comprehensive LC-MS-based quantitative analysis of fentanyl-like drugs in plasma and urine

Journal of Separation Science
Sarah CooremanKathleen Croes

Abstract

Fentanyl, norfentanyl, alfentanil, sufentanil, remifentanil and 3-methylfentanyl are potent, short-acting, synthetic narcotic analgesics that are not revealed in standard opiate immunoassays. In this article, a fully validated analytical method for the determination of these fentanyl-type compounds in plasma and urine is presented, consisting of a liquid-liquid extraction followed by a LC-MS/MS analysis using electrospray ionisation in the positive ionisation mode. Fentanyl-d(5) and norfentanyl-d(5) were used as internal standards. The lower LOQ in plasma and urine was 0.1 ng/mL for fentanyl, norfentanyl, alfentanil, remifentanil and 3-methylfentanyl, and 0.2 ng/mL for sufentanil. The method proved linear over a concentration range of 0.2-50 ng/mL for sufentanil and 0.1-50 ng/mL for all other analytes, with correlation coefficients of 0.998 or better. The analytical procedure showed excellent selectivity and precision (all CVs below 15%) for all analytes. Accuracy was good, except for sufentanil, where deviations of more than 15% from nominal concentrations were observed. No matrix effects were observed, and stability of stock and internal standard solutions was within acceptability limits.

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Citations

Nov 28, 2012·Journal of Medical Toxicology : Official Journal of the American College of Medical Toxicology·Michael C Milone
Feb 21, 2013·Journal of Analytical Toxicology·Thomas BergDag Helge Strand
Sep 24, 2013·Journal of Analytical Toxicology·Caroline PaulySerge Schneider
Mar 9, 2011·Journal of Pharmaceutical and Biomedical Analysis·Thomas KimEvan D Kharasch
Aug 5, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·K EckartR Urban
Sep 26, 2014·Medizinische Klinik, Intensivmedizin und Notfallmedizin·U WalterU Walther
Jan 29, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Gabriella RodaMichele Dei Cas
May 7, 2018·Forensic Sciences Research·Olaf H Drummer
Jul 7, 2020·Drug Testing and Analysis·Joshua KlingbergShanlin Fu
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marta TikhomirovBłażej Poźniak

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