A comprehensive ovine model of blood transfusion

Vox Sanguinis
G SimonovaY L Fung


The growing awareness of transfusion-associated morbidity and mortality necessitates investigations into the underlying mechanisms. Small animals have been the dominant transfusion model but have associated limitations. This study aimed to develop a comprehensive large animal (ovine) model of transfusion encompassing: blood collection, processing and storage, compatibility testing right through to post-transfusion outcomes. Two units of blood were collected from each of 12 adult male Merino sheep and processed into 24 ovine-packed red blood cell (PRBC) units. Baseline haematological parameters of ovine blood and PRBC cells were analysed. Biochemical changes in ovine PRBCs were characterized during the 42-day storage period. Immunological compatibility of the blood was confirmed with sera from potential recipient sheep, using a saline and albumin agglutination cross-match. Following confirmation of compatibility, each recipient sheep (n = 12) was transfused with two units of ovine PRBC. Procedures for collecting, processing, cross-matching and transfusing ovine blood were established. Although ovine red blood cells are smaller and higher in number, their mean cell haemoglobin concentration is similar to human red blood cells. Ov...Continue Reading


Mar 11, 1975·Proceedings of the Society for Experimental Biology and Medicine·R C GilesR P McCaffrey
Oct 1, 1987·International Journal of Oral and Maxillofacial Surgery·A G Bosanquet, A N Goss
Jan 1, 1997·Journal of Veterinary Internal Medicine·K J WardropK Mugnai
Oct 21, 2000·Lancet·Fiona HoustonC J Bostock
Nov 18, 2000·Genome Génome / Conseil National De Recherches Canada·T R Gregory
Dec 1, 1996·Manual Therapy·N. Timmerman, J. D. Boyling
Oct 22, 2002·The Journal of General Virology·Nora HunterFiona Houston
Jan 1, 1960·The American Journal of Physiology·S M WEISSMANN I BERLIN
Oct 6, 2005·Intensive Care Medicine·Michael Oppert, Achim Jörres
Apr 22, 2006·Journal of Applied Physiology·Thomas RiedelAndreas Schibler
Jul 29, 2006·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Mark A Popovsky
Oct 1, 1949·The Journal of Experimental Medicine·W F BALE, C L YUILE
Mar 20, 2008·Anesthesia and Analgesia·Hugh M SmithJuraj Sprung
Feb 25, 2009·Critical Care Medicine·George W MachiedoEdwin A Deitch
Jul 4, 2009·Transfusion·Christopher R GilsonJames C Zimring
Aug 12, 2009·Journal of Proteomics·David W GreeningRichard J Simpson
Oct 1, 2009·Lasers in Surgery and Medicine·Thomas M GlanzmannGeorges A Wagnières
Jan 22, 2010·Shock·Amy T MakleyTimothy A Pritts
Apr 13, 1946·British Medical Journal
Oct 21, 2010·Physiological Reviews·Yuansheng Gao, J Usha Raj
Feb 11, 2011·The Journal of Trauma·Susannah E NicholsonFruzsina K Johnson
May 10, 2011·Transfusion Medicine Reviews·Jacques LacroixABLE study group
Feb 3, 2012·Critical Care : the Official Journal of the Critical Care Forum·John-Paul TungYoke L Fung


Feb 13, 2016·Frontiers in Neurology·Judith BellapartJ F Fraser
Jan 28, 2014·British Journal of Haematology·Willy A FlegelHarvey G Klein
Mar 7, 2015·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Charles I McDonaldJohn F Fraser
Jul 25, 2019·Circulation·Alexander G BickVA Million Veteran Program
Apr 19, 2015·Current Opinion in Pediatrics·Kenneth E RemyHarvey G Klein
Nov 10, 2018·Intensive Care Medicine Experimental·Judith BellapartJ F Fraser

Related Concepts

Blood Grouping and Crossmatching
Blood Preservation
Blood Transfusion
Hematologic Tests
Dall Sheep
Collection of Blood Specimen for Laboratory Procedure

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.