A comprehensive proteome map of growing Bacillus subtilis cells

Proteomics
Christine EymannMichael Hecker

Abstract

The proteome of growing cells of Bacillus subtilis was analyzed in order to provide the basis for its application in microbial physiology. DNA arrays were used to calculate the number of genes transcribed in growing cells. From the 4100 B. subtilis genes, 2515 were actively transcribed in cells grown under standard conditions. From these genes 1544 proteins should be covered by our standard gel system pI 4-7. Using this standard gel system and supplementary zoom gels (pI 5.5-6.7, 5-6, 4.5-5.5, and 4-5) 693 proteins which are expressed in growing cells were detected that cover more than 40% of the vegetative proteome predicted for this region. Particularly broad coverage and thus comprehensive monitoring will be possible for central carbohydrate metabolism (glycolysis, pentose phosphate shunt, and citric acid cycle), amino acid synthesis pathways, purine and pyrimidine metabolism, fatty acid metabolism, and main cellular functions like replication, transcription, translation, and cell wall synthesis. Comparing the theoretical pI and Mr values with those experimentally determined a reasonable correlation was found for the majority of protein spots. By a color code outliers with dramatic deviations in charge or mass were visualize...Continue Reading

Citations

Aug 24, 2007·Applied Microbiology and Biotechnology·Matthias BerthJörg Bernhardt
Mar 17, 2005·Biochimica Et Biophysica Acta·Anthony W FrancisSrinivas Iyer
Jun 19, 2012·Journal of Proteome Research·Sven BielerSilke Meiners
Dec 22, 2012·Journal of Proteome Research·Vivianne J GoosensJan Maarten van Dijl
Jan 27, 2011·Nature Communications·Andreas OttoDörte Becher
Sep 18, 2010·The Journal of Biological Chemistry·Linda PasztorFriedrich Götz
Jan 27, 2012·The Journal of Biological Chemistry·Kaori KobayashiJunichi Sekiguchi
May 8, 2008·Molecular & Cellular Proteomics : MCP·Susanne WolffDörte Becher
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Garret SuenRoy D Welch
Oct 17, 2008·The Journal of Infectious Diseases·Gábor NagyJörg Hacker
Mar 13, 2009·Briefings in Functional Genomics & Proteomics·Carla M R Lacerda, Kenneth F Reardon
Mar 9, 2011·Antimicrobial Agents and Chemotherapy·Michaela WenzelJulia E Bandow
Aug 29, 2012·Antimicrobial Agents and Chemotherapy·Michaela WenzelJulia E Bandow
Jun 29, 2011·Antimicrobial Agents and Chemotherapy·Anna-Barbara HachmannJohn D Helmann
Mar 27, 2007·Journal of Bacteriology·Stephan FuchsSusanne Engelmann
Aug 5, 2008·Journal of Bacteriology·Christian KohlerSusanne Engelmann
Jun 15, 2007·Journal of Bacteriology·Julia MakhlinYair Aharonowitz
Oct 20, 2009·Journal of Bacteriology·Dierk-Christoph PötherMichael Hecker
May 18, 2010·Journal of Bacteriology·Mark J J B SibbaldJan Maarten van Dijl
Mar 18, 2008·Journal of Bacteriology·Cédric AbsalonSimone J Séror
May 4, 2005·Journal of Bacteriology·Daniel N WoodBettina A Buttaro
May 5, 2006·Journal of Bacteriology·Juliane OllingerJohn D Helmann
Jun 15, 2011·BMC Microbiology·Pilar Martinez-MoyaVíctor Cifuentes
May 20, 2009·BMC Microbiology·Kati SeidlBrigitte Berger-Bächi
Apr 9, 2008·Microbial Cell Factories·Jessica C ZweersJan Maarten van Dijl
Jun 1, 2010·Microbial Cell Factories·Britta JürgenPeter Neubauer
Jun 8, 2010·Proteome Science·Pradeep R DumpalaAttila Karsi
Jul 22, 2008·Chemistry Central Journal·Peter R JungblutHartmut Schlüter

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