A comprehensive rice transcript map containing 6591 expressed sequence tag sites

The Plant Cell
Jianzhong WuTakuji Sasaki

Abstract

To determine the chromosomal positions of expressed rice genes, we have performed an expressed sequence tag (EST) mapping project by polymerase chain reaction-based yeast artificial chromosome (YAC) screening. Specific primers designed from 6713 unique EST sequences derived from 19 cDNA libraries were screened on 4387 YAC clones and used for map construction in combination with genetic analysis. Here, we describe the establishment of a comprehensive YAC-based rice transcript map that contains 6591 EST sites and covers 80.8% of the rice genome. Chromosomes 1, 2, and 3 have relatively high EST densities, approximately twice those of chromosomes 11 and 12, and contain 41% of the total EST sites on the map. Most of the EST-dense regions are distributed on the distal regions of each chromosome arm. Genomic regions flanking the centromeres for most of the chromosomes have lower EST density. Recombination frequency in these regions is suppressed significantly. Our EST mapping also shows that 40% of the assigned ESTs occupy only approximately 21% of the entire genome. The rice transcript map has been a valuable resource for genetic study, gene isolation, and genome sequencing at the Rice Genome Research Program and should become an imp...Continue Reading

References

Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·A BarakatG Bernardi
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·F DongJ Jiang
Oct 23, 1998·Science·P DeloukasD R Bentley
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Feb 24, 2001·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·J YazakiS Kikuchi
Mar 28, 2001·Genome Génome / Conseil National De Recherches Canada·S SajiT Sasaki

❮ Previous
Next ❯

Citations

Sep 17, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hui WeiRajeev Arora
Apr 23, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Renate HornAlbert Glenn Abbott
Feb 16, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·X Y WanX P Guo
Jun 20, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K ShirasawaT Nishio
Dec 6, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Danting LiJianmin Wan
Nov 30, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Maiko MiyataNaoto Nitta
Jan 16, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Nils SteinAndreas Graner
Sep 8, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kenta ShirasawaTakeshi Nishio
Jan 26, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y Z XingQifa Zhang
Dec 6, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Goro IshikawaTakashi Matsumoto
Oct 23, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Julie OrjuelaMathias Lorieux
Mar 9, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Neha GujariaRajeev K Varshney
Feb 6, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shunzong NingTakao Komatsuda
Jul 10, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yubo ChenJianyi Zhao
Nov 2, 2005·Molecular Genetics and Genomics : MGG·Chia-Hsiung ChengTeh-Yuan Chow
Nov 12, 2005·Molecular Genetics and Genomics : MGG·I AshikawaK Sakata
Sep 28, 2005·Functional & Integrative Genomics·Joshua C JohnsonMrinal Bhave
Apr 2, 2003·Current Opinion in Plant Biology·Bin Han, Yongbiao Xue
Apr 2, 2003·Current Opinion in Plant Biology·Michel Delseny
Apr 2, 2003·Current Opinion in Plant Biology·Doreen Ware, Lincoln Stein
Nov 26, 2002·Nature·Qi FengBin Han
Nov 26, 2002·Nature·Takuji SasakiTakashi Gojobori
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kotaro MiuraMotoyuki Ashikari
Sep 16, 2011·Molecular Biology and Evolution·Jonathan M FlowersMichael D Purugganan
Dec 19, 2003·Nucleic Acids Research·Junshi YazakiShoshi Kikuchi
Apr 6, 2004·Nucleic Acids Research·Yu ZhangBin Han
Jul 9, 2004·Nucleic Acids Research·Mustafa EraymanKulvinder S Gill
Dec 20, 2007·Nucleic Acids Research·UNKNOWN Rice Annotation ProjectManuel Echeverria
Dec 14, 2006·Plant & Cell Physiology·Ji Hoon AhnJong Seob Lee
Mar 10, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Bin HanQifa Zhang
Mar 3, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jun YuHuanming Yang
Jan 14, 2004·Genome Research·Chengcang WuHong-Bin Zhang
Aug 20, 2005·Genome Research·C Robin BuellUNKNOWN Rice Chromosome 3 Sequencing Consortium
Dec 13, 2005·Genome Research·Lorinda K AndersonBrandon S Gaut
May 9, 2002·Genome Research·Qiang ZhaoBin Han
Jul 22, 2004·Plant Physiology·Ying-Jia ShenZhong-Nan Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.